Laboratory of Bioinspired Materials, Department of Science, University of Basilicata, Potenza, Italy.
PhD School of Science, University of Basilicata, Potenza, Italy.
J Biomed Mater Res A. 2019 Mar;107(3):535-544. doi: 10.1002/jbm.a.36568. Epub 2018 Nov 19.
Self-assembling peptide hydrogels (SAPHs) represent emerging cell cultures systems in several biomedical applications. The advantages of SAPHs are mainly ascribed to the absence of toxic chemical cross-linkers, the presence of ECM-like fibrillar structures and the possibility to produce hydrogels with a large range of different mechanical properties. We will present a two-component peptide system with tuneable mechanical properties, consisting of a small pentapeptide (SFFSF-NH , SA5N) that acts as a gelator and a larger 21-mer peptide (SFFSF-GVPGVGVPGVG-SFFSF, SA21) designed as a physical cross-linker. The hydrogels formed by different mixtures of the two peptides are made up mainly of antiparallel β-sheet nanofibers entangling in an intricate network. The effect of the addition of SA21 on the morphology of the hydrogels was investigated by atomic force microscopy and transmission electron microscopy and correlated to the mechanical properties of the hydrogel. Finally, the biocompatibility of the hydrogels using 2D cell cultures was tested. © 2018 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 535-544, 2019.
自组装肽水凝胶 (SAPHs) 在多种生物医学应用中代表了新兴的细胞培养系统。SAPHs 的优点主要归因于不存在有毒化学交联剂、存在类似细胞外基质的纤维状结构以及能够生产具有广泛不同机械性能的水凝胶的可能性。我们将介绍一种具有可调机械性能的双组分肽系统，该系统由一种小五肽 (SFFSF-NH 2, SA5N) 组成，作为凝胶剂，和一种较大的 21 肽 (SFFSF-GVPGVGVPGVG-SFFSF, SA21) 设计为物理交联剂。由两种肽的不同混合物形成的水凝胶主要由反平行 β-折叠纳米纤维交织成错综复杂的网络组成。通过原子力显微镜和透射电子显微镜研究了 SA21 的添加对水凝胶形态的影响，并将其与水凝胶的机械性能相关联。最后，使用二维细胞培养测试了水凝胶的生物相容性。 2018 年作者。杂志生物医学材料研究部分 A 由 Wiley 期刊出版，公司出版。J Biomed Mater Res Part A: 107A: 535-544, 2019。
