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Molecular consequences of truncations of the first exon for in vitro splicing of yeast actin pre-mRNA.

作者信息

Duchêne M, Löw A, Schweizer A, Domdey H

机构信息

Laboratorium für Molekulare Biologie, Genzentrum-der-Ludwig-Maximilians-Universität, München, Martinsried, FRG.

出版信息

Nucleic Acids Res. 1988 Aug 11;16(15):7233-9. doi: 10.1093/nar/16.15.7233.

DOI:10.1093/nar/16.15.7233
PMID:3045753
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338405/
Abstract

A defined minimum length of the first exon is required for the generation of spliced products from a synthetic yeast actin mRNA-precursor in vitro. If the first exon is 1, 2, 3 or 5 nucleotides long, only the first step of the splicing reaction can take place. A transcript starting with the first nucleotide of the intron does not get converted into any of the normally obtained splicing products or intermediates. On the other hand, spliceosome assembly does not depend on the presence of a first exon.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee3e/338405/49a172f99ae4/nar00157-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee3e/338405/49a172f99ae4/nar00157-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee3e/338405/49a172f99ae4/nar00157-0029-a.jpg

相似文献

1
Molecular consequences of truncations of the first exon for in vitro splicing of yeast actin pre-mRNA.
Nucleic Acids Res. 1988 Aug 11;16(15):7233-9. doi: 10.1093/nar/16.15.7233.
2
Exon definition may facilitate splice site selection in RNAs with multiple exons.外显子定义可能有助于具有多个外显子的RNA中剪接位点的选择。
Mol Cell Biol. 1990 Jan;10(1):84-94. doi: 10.1128/mcb.10.1.84-94.1990.
3
Yeast mRNA splicing in vitro.体外酵母mRNA剪接
J Biol Chem. 1985 Nov 25;260(27):14780-92.
4
Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.间隔序列的大小和位置对于酿酒酵母中前体mRNA的剪接效率至关重要。
Nucleic Acids Res. 1985 Jun 11;13(11):3791-804. doi: 10.1093/nar/13.11.3791.
5
The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.“剪接体”：酵母前体信使核糖核酸在剪接依赖性反应中与一个40S复合体结合。
Science. 1985 May 24;228(4702):963-7. doi: 10.1126/science.3890181.
6
Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome.保守内含子序列中的突变会影响酵母剪接途径中的多个步骤，尤其是剪接体的组装。
EMBO J. 1986 Jul;5(7):1683-95. doi: 10.1002/j.1460-2075.1986.tb04412.x.
7
Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.套索结构是酵母前体mRNA剪接过程中的体内中间体。
Cell. 1984 Dec;39(3 Pt 2):611-21. doi: 10.1016/0092-8674(84)90468-9.
8
Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.构建一个酵母肌动蛋白基因内含子缺失突变体，该突变体在剪接方面存在缺陷，导致转化酵母细胞中前体RNA积累。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3493-7. doi: 10.1073/pnas.79.11.3493.
9
Impairment of yeast pre-mRNA splicing by potential secondary structure-forming sequences near the conserved branchpoint sequence.保守分支点序列附近潜在的二级结构形成序列对酵母前体mRNA剪接的损害。
Nucleic Acids Res. 1988 Nov 25;16(22):10413-23. doi: 10.1093/nar/16.22.10413.
10
Interaction of the yeast DExH-box RNA helicase prp22p with the 3' splice site during the second step of nuclear pre-mRNA splicing.酵母DExH盒RNA解旋酶prp22p在核内前体mRNA剪接第二步过程中与3'剪接位点的相互作用。
Nucleic Acids Res. 2000 Mar 15;28(6):1313-21. doi: 10.1093/nar/28.6.1313.

引用本文的文献

1
5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly.人类剪接体内的5'外显子相互作用为外显子连接复合体的结构和组装建立了一个框架。
Genes Dev. 2002 Nov 1;16(21):2778-91. doi: 10.1101/gad.1030602.
2
The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.外显子-外显子连接复合体为参与mRNA输出和无义介导的mRNA降解的因子提供了一个结合平台。
EMBO J. 2001 Sep 3;20(17):4987-97. doi: 10.1093/emboj/20.17.4987.
3
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.

本文引用的文献

1
Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.构建一个酵母肌动蛋白基因内含子缺失突变体，该突变体在剪接方面存在缺陷，导致转化酵母细胞中前体RNA积累。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3493-7. doi: 10.1073/pnas.79.11.3493.
2
Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.酿酒酵母肌动蛋白基因的分离与测序。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6. doi: 10.1073/pnas.77.7.3912.
3
Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.
剪接体在mRNA外显子-外显子连接点上游20-24个核苷酸处沉积多种蛋白质。
EMBO J. 2000 Dec 15;19(24):6860-9. doi: 10.1093/emboj/19.24.6860.
4
Trans-complementation of the second step of pre-mRNA splicing by exogenous 5' exons.外源性5'外显子对前体mRNA剪接第二步的反式互补作用。
RNA. 1999 Jul;5(7):876-82. doi: 10.1017/s1355838299990544.
5
In vitro generation of a circular exon from a linear pre-mRNA transcript.从线性前体mRNA转录本体外生成环状外显子。
Nucleic Acids Res. 1996 Apr 1;24(7):1260-6. doi: 10.1093/nar/24.7.1260.
6
Splicing of a circular yeast pre-mRNA in vitro.体外对环状酵母前体mRNA进行剪接。
Nucleic Acids Res. 1995 Apr 11;23(7):1133-9. doi: 10.1093/nar/23.7.1133.
7
Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe.将功能性人工内含子导入粟酒裂殖酵母天然无内含子的ura4基因中。
Mol Cell Biol. 1989 Apr;9(4):1526-35. doi: 10.1128/mcb.9.4.1526-1535.1989.
8
Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro.
EMBO J. 1991 Dec;10(12):3869-75. doi: 10.1002/j.1460-2075.1991.tb04956.x.
9
The intron of the yeast actin gene contains the promoter for an antisense RNA.酵母肌动蛋白基因的内含子包含一个反义RNA的启动子。
Curr Genet. 1990 Mar;17(3):269-73. doi: 10.1007/BF00312620.
点突变确定了保守的、内含于内含子中的TACTAAC盒是酵母中一个必需的剪接信号序列。
Cell. 1984 Mar;36(3):645-53. doi: 10.1016/0092-8674(84)90344-1.
4
tRNA gene transcription in yeast: effects of specified base substitutions in the intragenic promoter.酵母中转运RNA基因的转录：基因内启动子中特定碱基替换的影响
Cell. 1983 Nov;35(1):117-25. doi: 10.1016/0092-8674(83)90214-3.
5
Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.套索RNA作为信使RNA前体剪接过程中的中间体和产物。
Science. 1984 Aug 31;225(4665):898-903. doi: 10.1126/science.6206566.
6
A minimal intron length but no specific internal sequence is required for splicing the large rabbit beta-globin intron.剪接大的兔β-珠蛋白内含子时，需要最小内含子长度，但不需要特定的内部序列。
Cell. 1984 Jul;37(3):915-25. doi: 10.1016/0092-8674(84)90426-4.
7
Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.套索结构是酵母前体mRNA剪接过程中的体内中间体。
Cell. 1984 Dec;39(3 Pt 2):611-21. doi: 10.1016/0092-8674(84)90468-9.
8
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
9
Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.在体外前体mRNA剪接过程中，完整内含子作为一种新型套索结构被切除。
Cell. 1984 Aug;38(1):317-31. doi: 10.1016/0092-8674(84)90553-1.
10
Cleavage of 5' splice site and lariat formation are independent of 3' splice site in yeast mRNA splicing.在酵母mRNA剪接过程中，5'剪接位点的切割和套索结构的形成与3'剪接位点无关。
Nature. 1985;317(6039):735-7. doi: 10.1038/317735a0.