Martins Guilherme De Caro, de Oliveira Melo Júnior Otoni Alves, Botoni Larissa Silveira, Nogueira Marina Moller, da Costa Val Adriane Pimenta, Blanco Benito Soto, Dutra Walderez Ornelas, Giunchetti Rodolfo Cordeiro, Melo Marília Martins, da Silveira Lemos Denise
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, São Luiz, CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil.
Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, São Luiz, CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil.
Vet Immunol Immunopathol. 2018 Nov;205:58-64. doi: 10.1016/j.vetimm.2018.10.009. Epub 2018 Oct 23.
Canine atopic dermatitis (CAD) is a chronic, pruritic, genetic, and inflammatory disease. Its pathogenesis is very complex and involves skin barrier defects and immune system dysfunction. This study aimed to investigate hematological, biochemical, clinical, and immunological parameters to contribute to the identification of biomarkers applied to CAD. The results of the analysis on hematologic and clinical parameters showed increased neutrophil numbers and decreased lymphocyte counts. The ex vivo immunophenotyping of leukocytes demonstrated increased counts of circulating neutrophils, in addition to a high frequency of CD4 T-cells and elevated CD4/CD8 T-cell ratio, which were the hallmark of atopic animals. Moreover, atopic dogs presented a mixed immune response, displaying both CD4 and CD8 T-cell subsets as relevant sources of IFN-γ and IL-4 cytokines. The morbidity analyzed by the CADESI index demonstrated that CAD severity is related to the low frequency of circulating CD14 monocytes, CD21 B-cells, and CD8 T-cells. The reported biomarkers would be useful in CAD monitoring for treatment and prognosis analysis.
犬特应性皮炎（CAD）是一种慢性、瘙痒性、遗传性炎症性疾病。其发病机制非常复杂，涉及皮肤屏障缺陷和免疫系统功能障碍。本研究旨在调查血液学、生化、临床和免疫学参数，以有助于识别应用于CAD的生物标志物。血液学和临床参数分析结果显示中性粒细胞数量增加，淋巴细胞计数减少。白细胞的体外免疫表型分析表明，循环中性粒细胞数量增加，此外，CD4 T细胞计数高，CD4/CD8 T细胞比值升高，这是特应性动物的标志。此外，特应性犬呈现混合免疫反应，显示CD4和CD8 T细胞亚群均为IFN-γ和IL-4细胞因子的相关来源。通过CADESI指数分析的发病率表明，CAD严重程度与循环CD14单核细胞、CD21 B细胞和CD8 T细胞的低频率有关。所报道的生物标志物将有助于CAD的治疗监测和预后分析。
