Cai Congxi, Yuan Wenxin, Miao Huiying, Deng Mingdan, Wang Mengyu, Lin Jiayao, Zeng Wei, Wang Qiaomei
State Agriculture Ministry Laboratory of Horticultural Crop Growth and Development, Hangzhou, China.
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou, China.
Front Plant Sci. 2018 Nov 6;9:1599. doi: 10.3389/fpls.2018.01599. eCollection 2018.
R2R3-MYB transcription factor MYB51 is known to control indole glucosinolate (indole GSL) biosynthesis in . Here, two copies of have been isolated in Chinese kale ( var. Bailey), designated and , which exhibit overlapping but distinct expression levels among different organs and respond to signaling molecules in a similar pattern. It has been demonstrated a structural and functional conservation between BoaMYB51s and AtMYB51 by phylogenetic analysis, complementation studies and transient expression assay. To further investigate the transcriptional mechanism, we identified the transcriptional activation domain (TAD) and putative interacting proteins of BoaMYB51s by means of yeast () two hybrid. Using tobacco () transient expression assay, we confirmed that the carboxy-end is required for transcriptional activation activity of BoaMYB51s. In addition, several BoaMYB51-interacting proteins have been identified by yeast two-hybrid screening. These results provide important insights into the molecular mechanisms by which MYB51 transcriptionally regulates indole GSL biosynthesis.
已知R2R3-MYB转录因子MYB51可调控[具体植物名称未给出]中吲哚硫代葡萄糖苷（吲哚GSL）的生物合成。在此，在中国芥蓝（var. Bailey）中分离出了两个[具体基因名称未给出]的拷贝，命名为[具体名称未给出]和[具体名称未给出]，它们在不同器官中表现出重叠但不同的表达水平，并且以相似模式对信号分子作出反应。通过系统发育分析、互补研究和瞬时表达分析，已证明BoaMYB51s与AtMYB51之间存在结构和功能上的保守性。为了进一步研究转录机制，我们通过酵母（）双杂交鉴定了BoaMYB51s的转录激活结构域（TAD）和假定的相互作用蛋白。利用烟草（）瞬时表达分析，我们证实了羧基末端是BoaMYB51s转录激活活性所必需的。此外，通过酵母双杂交筛选鉴定了几种与BoaMYB51相互作用的蛋白。这些结果为MYB51转录调控吲哚GSL生物合成的分子机制提供了重要见解。
