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控制白菜硫代葡萄糖苷生物合成的三个BrMYB28转录因子的功能分析

Functional analysis of three BrMYB28 transcription factors controlling the biosynthesis of glucosinolates in Brassica rapa.

作者信息

Seo Mi-Suk, Jin Mina, Chun Jin-Hyuk, Kim Sun-Ju, Park Beom-Seok, Shon Seong-Han, Kim Jung Sun

机构信息

Genomics Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, Rural Development Administration (RDA), Wansan-gu, Jeonju, Korea.

Department of Biological Environment and Chemistry, College of Agriculture and Life Science, Chungnam National University, Yuseong-gu, Daejeon, Korea.

出版信息

Plant Mol Biol. 2016 Mar;90(4-5):503-16. doi: 10.1007/s11103-016-0437-z. Epub 2016 Jan 28.

DOI:10.1007/s11103-016-0437-z
PMID:26820138
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4766241/
Abstract

Glucosinolates (GSLs) are secondary metabolites that have anticarcinogenic activity and play defense roles in plants of the Brassicaceae family. MYB28 is known as a transcription factor that regulates aliphatic GSL biosynthesis in Arabidopsis thaliana. Brassicaceae plants have three orthologous copies of AtMYB28 derived from recent genome triplication. These BrMYB28 genes have a high level of sequence homology, with 81-87% similarities in the coding DNA sequence compared to Arabidopsis. Overexpression of three paralogous BrMYB28 genes in transgenic Chinese cabbage increased the total GSL content in all T1 generation plants and in two inbred lines of homozygous T2 plants. The highest total GSL contents were detected in homozygous T2 lines overexpressing BrMYB28.1, which showed an approximate fivefold increase compared to that of nontransgenic plants. The homozygous T2 lines with overexpressed BrMYB28.1 also showed an increased content of aliphatic, indolic, and aromatic GSLs compared to that of nontransgenic plants. Furthermore, all of the three BrMYB28 genes were identified as negative regulators of BrAOP2 and positive regulators of BrGSL-OH in the homozygous T2 lines. These data indicate the regulatory mechanism of GSL biosynthesis in B. rapa is unlike that in A. thaliana. Our results will provide useful information for elucidating the regulatory mechanism of GSL biosynthesis in polyploid plants.

摘要

硫代葡萄糖苷（GSLs）是次生代谢产物，具有抗癌活性，并在十字花科植物中发挥防御作用。MYB28是一种已知的转录因子，可调节拟南芥中脂肪族GSL的生物合成。十字花科植物有三个源自近期基因组三倍化的AtMYB28直系同源拷贝。这些BrMYB28基因具有高度的序列同源性，与拟南芥相比，编码DNA序列的相似度为81-87%。在转基因大白菜中过表达三个旁系同源的BrMYB28基因，增加了所有T1代植株以及两个纯合T2植株自交系中的总GSL含量。在过表达BrMYB28.1的纯合T2系中检测到最高的总GSL含量，与非转基因植物相比，增加了约五倍。与非转基因植物相比，过表达BrMYB28.1的纯合T2系中脂肪族、吲哚族和芳香族GSLs的含量也有所增加。此外，在纯合T2系中，所有三个BrMYB28基因均被鉴定为BrAOP2的负调控因子和BrGSL-OH的正调控因子。这些数据表明，甘蓝型油菜中GSL生物合成的调控机制与拟南芥不同。我们的研究结果将为阐明多倍体植物中GSL生物合成的调控机制提供有用信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/12fd5be30507/11103_2016_437_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/a9e2bcfadcd7/11103_2016_437_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/3ec844b97607/11103_2016_437_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/7187dabdb6ff/11103_2016_437_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/84451849859b/11103_2016_437_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/2e22d13392b9/11103_2016_437_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/b0fc54eb03a1/11103_2016_437_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/e1d44335a5c4/11103_2016_437_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/12fd5be30507/11103_2016_437_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/a9e2bcfadcd7/11103_2016_437_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/3ec844b97607/11103_2016_437_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/7187dabdb6ff/11103_2016_437_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/84451849859b/11103_2016_437_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/2e22d13392b9/11103_2016_437_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/b0fc54eb03a1/11103_2016_437_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/e1d44335a5c4/11103_2016_437_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9289/4766241/12fd5be30507/11103_2016_437_Fig8_HTML.jpg

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