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NADP 依赖性苹果酸酶 1 参与[具体植物名称未给出]中的脱落酸反应。

NADP-Dependent Malic Enzyme 1 Participates in the Abscisic Acid Response in .

作者信息

Arias Cintia L, Pavlovic Tatiana, Torcolese Giuliana, Badia Mariana B, Gismondi Mauro, Maurino Verónica G, Andreo Carlos S, Drincovich María F, Gerrard Wheeler Mariel C, Saigo Mariana

机构信息

Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Rosario, Argentina.

Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, Cluster of Excellence on Plant Sciences, Düsseldorf, Germany.

出版信息

Front Plant Sci. 2018 Nov 6;9:1637. doi: 10.3389/fpls.2018.01637. eCollection 2018.

DOI:10.3389/fpls.2018.01637
PMID:30459802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6232891/
Abstract

possesses three cytosolic (NADP-ME1-3) and one plastidic (NADP-ME4) NADP-dependent malic enzymes. NADP-ME2 and -ME4 show constitutive expression, in contrast to NADP-ME1 and -ME3, which are restricted to particular tissues. Here, we show that NADP-ME1 transcript and protein were almost undetectable during normal vegetative growth, but gradually increased and reached levels higher than those of the other isoforms in the latest stages of seed development. Accordingly, in knockout mature seeds the total NADP-ME activity was significantly lower than in wild type mature seeds. The phenotypic analysis of plants indicated alterations of seed viability and germination. Besides, the treatment with abscisic acid (ABA), NaCl and mannitol specifically induced the accumulation of NADP-ME1 in seedlings. In line with this, plants show a weaker response of primary and lateral root length and stomatal opening to the presence of ABA. The results suggest that NADP-ME1 plays a specialized role, linked to ABA signaling during the seed development as well as in the response to water deficit stress.

摘要

具有三种胞质（NADP - ME1 - 3）和一种质体（NADP - ME4）依赖NADP的苹果酸酶。与局限于特定组织的NADP - ME1和 - ME3不同，NADP - ME2和 - ME4呈现组成型表达。在此，我们表明在正常营养生长期间，NADP - ME1转录本和蛋白质几乎检测不到，但在种子发育的后期逐渐增加并达到高于其他同工型的水平。因此，在基因敲除的成熟种子中，总NADP - ME活性显著低于野生型成熟种子。对植物的表型分析表明种子活力和萌发发生了改变。此外，用脱落酸（ABA）、NaCl和甘露醇处理可特异性诱导幼苗中NADP - ME1的积累。与此一致，植物对ABA存在时主根和侧根长度以及气孔开放的反应较弱。结果表明，NADP - ME1在种子发育过程中以及对水分亏缺胁迫的反应中发挥与ABA信号传导相关的特殊作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/171483babb18/fpls-09-01637-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/920ee49fbba2/fpls-09-01637-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/cfeff59f65a8/fpls-09-01637-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/2a1a71a7452c/fpls-09-01637-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/59ec42bda7c9/fpls-09-01637-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/428f8bd42207/fpls-09-01637-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/171483babb18/fpls-09-01637-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/920ee49fbba2/fpls-09-01637-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/cfeff59f65a8/fpls-09-01637-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/2a1a71a7452c/fpls-09-01637-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/59ec42bda7c9/fpls-09-01637-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/428f8bd42207/fpls-09-01637-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e7b/6232891/171483babb18/fpls-09-01637-g006.jpg

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