You Sixian, Sun Yi, Chaney Eric J, Zhao Youbo, Chen Jianxin, Boppart Stephen A, Tu Haohua
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Biomed Opt Express. 2018 Oct 5;9(11):5253-5268. doi: 10.1364/BOE.9.005253. eCollection 2018 Nov 1.
Tumor-adjacent "normal" tissue constitutes a peri-tumoral field that affects early cancer detection, risk assessment, surgical decision, and postoperative surveillance. Modern genetic analysis has revealed valuable information from this field, but without the spatial resolution of optical microscopy to understand the vital microenvironments that surround individual cells. Rapidly advanced optical imaging techniques free of labor-intensive sample preparation, despite great promise to perform slide-free imaging of cell and shift the histology-centered cancer diagnostic paradigm, have lacked compatible and complementary histochemical imaging of cell or to interrogate the peri-tumoral field. In the first part (Part I) of this two-part series study, we developed a technique of slide-free virtual histochemistry to phenotype various cells in animal and human tissue. Here, in the second part (Part II) of this two-part series study, we employ this technique to examine various peri-tumoral fields and produce the volumetric histochemical evidence of field cancerization consistent with the structural changes at larger spatial scales. We also link the field cancerization with cancer dormancy in a significant portion of breast cancer patients.
肿瘤邻近的“正常”组织构成了一个肿瘤周围区域，该区域会影响早期癌症检测、风险评估、手术决策和术后监测。现代基因分析已从该区域揭示了有价值的信息，但缺乏光学显微镜的空间分辨率来了解单个细胞周围的重要微环境。快速发展的光学成像技术无需进行劳动密集型的样本制备，尽管有望实现无玻片细胞成像并改变以组织学为中心的癌症诊断模式，但一直缺乏兼容且互补的细胞或组织化学成像来研究肿瘤周围区域。在这个两部分系列研究的第一部分（第一部分）中，我们开发了一种无玻片虚拟组织化学技术，用于对动物和人类组织中的各种细胞进行表型分析。在此，在这个两部分系列研究的第二部分（第二部分）中，我们运用该技术检查各种肿瘤周围区域，并生成与更大空间尺度上的结构变化一致的场癌变的体积组织化学证据。我们还将场癌变与相当一部分乳腺癌患者的癌症休眠联系起来。
