Kellman Michael, Chen Michael, Phillips Zachary F, Lustig Michael, Waller Laura
Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, CA 94720, USA.
Applied Science and Technology, University of California, Berkeley, CA 94720, USA.
Biomed Opt Express. 2018 Oct 15;9(11):5456-5466. doi: 10.1364/BOE.9.005456. eCollection 2018 Nov 1.
The temporal resolution of quantitative phase imaging with Differential Phase Contrast (DPC) is limited by the requirement for multiple illumination-encoded measurements. This inhibits imaging of fast-moving samples. We present a computational approach to model and correct for non-rigid sample motion during the DPC acquisition in order to improve temporal resolution to that of a single-shot method and enable imaging of motion dynamics at the framerate of the sensor. Our method relies on the addition of a simultaneously-acquired color-multiplexed reference signal to enable non-rigid registration of measurements prior to phase retrieval. We show experimental results where we reduce motion blur from fast-moving live biological samples.
差分相衬（DPC）定量相成像的时间分辨率受多次照明编码测量要求的限制。这阻碍了对快速移动样本的成像。我们提出一种计算方法，用于在DPC采集期间对非刚性样本运动进行建模和校正，以便将时间分辨率提高到单次方法的水平，并能够以传感器的帧率对运动动力学进行成像。我们的方法依赖于添加同时采集的颜色多路复用参考信号，以便在相位检索之前对测量进行非刚性配准。我们展示了减少快速移动的活生物样本运动模糊的实验结果。
