Yallappa S, Manjanna J, Dhananjaya B L, Vishwanatha U, Ravishankar B, Gururaj H, Niranjana P, Hungund B S
1Department of Industrial Chemistry, Kuvempu University, Shankaraghatta, Shimoga-Dist, 577 451 India.
2Department of Chemistry, Rani Channamma University, Belagavi, 591 156 India.
Nanomicro Lett. 2016;8(2):120-130. doi: 10.1007/s40820-015-0066-0. Epub 2015 Oct 15.
Nanomedicine is an emerging field concerned with the use of precision engineered nanomaterials, which leads to the development of novel remedial and diagnostic modalities for human use. In this study, Cu(NO) and AgNO precursors were reduced to copper nanoparticles (CuNPs) and silver nanoparticles (AgNPs) using bark extracts under microwave irradiation in the presence of well-dispersed multi-walled carbon nanotubes (MWCNTs) in aqueous medium. The formation of CuNPs or AgNPs and their functionalization with MWCNTs via bioactive molecules of plant extract were evidenced from UV-Vis spectra, XRD, FTIR, FESEM, EDX, and TEM images. The phytochemically functionalized Cu-MWCNTs and Ag-MWCNTs nanomaterials showed enhanced biocide activity, and the inhibitory activity for bacteria was higher than that of fungus. Furthermore, these biohybrid nanomaterials are non-toxic to normal epithelial cells (Vero), whereas they are highly toxic for tested human cancer cells of MDA-MB-231, HeLa, SiHa, and Hep-G2. The cell viability was found to decrease with the increasing dose from 10 to 50 µg mL, as well as incubation time from 24 to 72 h. For instance, the cell viability was found to be ~91 % for normal Vero cells and ~76 % for cancer cells for lower dose of 10 µg mL.
纳米医学是一个新兴领域，涉及使用精确工程的纳米材料，这导致了用于人类的新型治疗和诊断方式的发展。在本研究中，在水介质中，在充分分散的多壁碳纳米管（MWCNTs）存在下，使用树皮提取物在微波辐射下将硝酸铜（Cu(NO)）和硝酸银（AgNO）前体还原为铜纳米颗粒（CuNPs）和银纳米颗粒（AgNPs）。紫外可见光谱、X射线衍射、傅里叶变换红外光谱、场发射扫描电子显微镜、能谱和透射电子显微镜图像证明了CuNPs或AgNPs的形成以及它们通过植物提取物的生物活性分子与MWCNTs的功能化。植物化学功能化的Cu-MWCNTs和Ag-MWCNTs纳米材料表现出增强的杀生物活性，并且对细菌的抑制活性高于对真菌的抑制活性。此外，这些生物杂化纳米材料对正常上皮细胞（Vero）无毒，而对测试的MDA-MB-231、HeLa、SiHa和Hep-G2人癌细胞具有高毒性。发现细胞活力随着剂量从10增加到50 μg/mL以及孵育时间从24增加到72小时而降低。例如，对于10 μg/mL的较低剂量，正常Vero细胞的细胞活力约为91%，癌细胞的细胞活力约为76%。
