Gulaia N M, Bezdrobnyĭ Iu V, Gavriliuk E S, Lutsik M D, Peredereĭ O F
Ukr Biokhim Zh (1978). 1988 May-Jun;60(3):3-10.
The presence of insulin receptors in neuroblastoma C 1300 N18 cells was shown by the method of the [125I]insulin binding with cells as well as by the electron-cytochemical methods based on the analysis of the binding of colloid gold-labelled insulin and of agglutinin of wheat embryos with the surface of plasma membrane. It is established that the number and distribution of insulin receptors depend on the functional state of cells. Expression of receptors on the membrane increases after 5'-deoxyuridine-induced differentiation of cells. Due to changes in the lipid composition of cells caused by the incubation with lecithin-cholesterol liposomes (an increase in the content of cholesterol and its esters, as well as of unsaturated fatty acids and appearance of lysolecithin) the quantity of insulin receptors decreases and cell membranes are damaged. The lowering insulin regulation is not observed for insulin receptors of the neuroblastoma C 1300 N18 cells.
通过[125I]胰岛素与细胞结合的方法以及基于分析胶体金标记胰岛素和小麦胚凝集素与质膜表面结合的电子细胞化学方法，证实了神经母细胞瘤C 1300 N18细胞中存在胰岛素受体。已确定胰岛素受体的数量和分布取决于细胞的功能状态。5'-脱氧尿苷诱导细胞分化后，膜上受体的表达增加。由于用卵磷脂 - 胆固醇脂质体孵育导致细胞脂质组成发生变化（胆固醇及其酯类含量增加，不饱和脂肪酸含量增加以及溶血卵磷脂出现），胰岛素受体数量减少且细胞膜受损。未观察到神经母细胞瘤C 1300 N18细胞的胰岛素受体的胰岛素调节作用降低。
