Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China.
Molecules. 2018 Nov 20;23(11):3026. doi: 10.3390/molecules23113026.
Chinese pickled cabbage is a traditional fermented food that contains abundant microbes produced during the process of fermentation. In this work, an in vivo animal study was conducted to investigate the effects of a newly isolated lactic acid bacterium ( CQPC11, LP-CQPC11) on d-galactose-induced oxidation and aging in mice. Analysis of the serum and tissue samples of these mice using molecular biology approaches showed that LP-CQPC11 suppressed the decrease in thymus, brain, heart, liver, spleen, and kidney indices caused by oxidation and aging. Furthermore, LP-CQPC11 increased the levels of SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), and GSH (glutathione), whereas it reduced the levels of NO (nitric oxide) and MDA (malondialdehyde) in the serum, liver, and spleen of oxidation and aging mouse models. Pathological observation indicated that LP-CQPC11 alleviated the damage caused by oxidation and aging on the liver and spleen of mice. qPCR analysis indicated that LP-CQPC11 effectively upregulated the expression of (neuronal nitric oxide synthase), (endothelial nitric oxide synthase), (cuprozinc-superoxide dismutase), (manganese superoxide dismutase), (catalase), (heme oxygenase-1), (nuclear factor-erythroid 2 related factor 2), γ (γ-glutamylcysteine synthetase), and (NAD(P)H dehydrogenase [quinone] 1), but downregulated the expression of (inducible nitric oxide synthase) in the mouse liver and spleen. Western blot analysis showed that LP-CQPC11 effectively upregulated SOD1 (), SOD2 (), CAT, GSH1 (c-glutamylcysteine synthetase), and GSH2 (glutathione synthetase) protein expression in mouse liver and spleen tissues. These findings suggest that LP-CQPC11 can effectively prevent d-galactose-induced oxidation and aging in mice, and the effect is even better than that of the commonly used subsp. (LDSB) and vitamin C in the industry. Thus, LP-CQPC11 may be potentially employed as a probiotic strain.
中国泡菜是一种传统的发酵食品，含有丰富的微生物，这些微生物是在发酵过程中产生的。本工作采用体内动物实验研究了一株新分离的乳酸杆菌（CQPC11，LP-CQPC11）对 D-半乳糖诱导的氧化和衰老小鼠的影响。采用分子生物学方法分析这些小鼠的血清和组织样本表明，LP-CQPC11 抑制了氧化和衰老引起的胸腺、脑、心、肝、脾、肾指数下降。此外，LP-CQPC11 提高了 SOD（超氧化物歧化酶）、GSH-Px（谷胱甘肽过氧化物酶）和 GSH（谷胱甘肽）的水平，同时降低了氧化和衰老小鼠模型血清、肝脏和脾脏中 NO（一氧化氮）和 MDA（丙二醛）的水平。病理观察表明，LP-CQPC11 减轻了氧化和衰老对小鼠肝脏和脾脏的损伤。qPCR 分析表明，LP-CQPC11 有效地上调了（神经元型一氧化氮合酶）、（内皮型一氧化氮合酶）、（铜锌-超氧化物歧化酶）、（锰超氧化物歧化酶）、（过氧化氢酶）、（血红素加氧酶-1）、（核因子-红细胞 2 相关因子 2）、γ（γ-谷氨酰半胱氨酸合成酶）和（NAD（P）H 脱氢酶[醌]1）的表达，但下调了（诱导型一氧化氮合酶）在小鼠肝脏和脾脏中的表达。Western blot 分析表明，LP-CQPC11 有效地上调了 SOD1（）、SOD2（）、CAT、GSH1（γ-谷氨酰半胱氨酸合成酶）和 GSH2（谷胱甘肽合成酶）在小鼠肝脏和脾脏组织中的蛋白表达。这些发现表明，LP-CQPC11 能有效预防 D-半乳糖诱导的氧化和衰老小鼠，其效果甚至优于行业常用的亚种。（LDSB）和维生素 C。因此，LP-CQPC11 可能作为一种潜在的益生菌菌株。
