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格雷夫斯眼眶病患者泪液中的氧化应激标志物及其与临床活动评分的相关性。

Oxidative stress markers in tears of patients with Graves' orbitopathy and their correlation with clinical activity score.

作者信息

Choi Won, Li Ying, Ji Yong Sok, Yoon Kyung Chul

机构信息

Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Medical School and Hospital, 42 Jebong-ro, Dong-gu, Gwangju, 61469, South Korea.

出版信息

BMC Ophthalmol. 2018 Nov 21;18(1):303. doi: 10.1186/s12886-018-0969-x.

Abstract

BACKGROUND

To investigate the concentrations of oxidative stress markers, 8-hydroxy-2'-deoxyquanosine (8-OHdG) and malondialdehyde (MDA), in tears and their correlation with the clinical activity score (CAS) in patients with Graves' orbitopathy (GO) according to disease activity.

METHODS

We recruited 27 participants with inactive stage GO, 35 participants with active stage GO, and 25 healthy controls without GO. The tear concentrations of 8-OHdG and MDA were determined by enzyme-linked immunosorbent assay. The correlation between CAS and the concentrations of tear 8-OHdG and MDA were analyzed according to the disease activity in the GO patients.

RESULTS

The levels of 8-OHdG and MDA were 56.30 ± 16.81 ng/mL and 5.39 ± 1.31 pmol/mg, respectively, in the control subjects, and 123.46 ± 22.67 ng/mL and 13.59 ± 3.93 pmol/mg, respectively, in patients with inactive stage GO, and 215.14 ± 35.61 ng/mL and 22.52 ± 4.63 pmol/mg, in patients with active stage GO. The mean concentrations of 8-OHdG and MDA were higher in patients with inactive and active stage GO compared with the control group (all P < 0.001). Furthermore, in the active stage group, tear concentrations of 8-OHdG and MDA were higher than those in the inactive stage group (all P < 0.001). The level of 8-OHdG (r = 0.676, P < 0.001) and MDA (r = 0.506, P = 0.002) correlated with CAS in the active stage GO group.

CONCLUSIONS

The concentrations of 8-OHdG and MDA in tears increased in patients with GO, especially in those in the active stage. In patients with active stage GO, CAS correlated significantly with the tear 8-OHdG and MDA levels.

摘要

背景

根据疾病活动度,研究格雷夫斯眼眶病(GO)患者泪液中氧化应激标志物8-羟基-2'-脱氧鸟苷(8-OHdG)和丙二醛(MDA)的浓度及其与临床活动评分(CAS)的相关性。

方法

我们招募了27例非活动期GO患者、35例活动期GO患者和25例无GO的健康对照者。采用酶联免疫吸附测定法测定泪液中8-OHdG和MDA的浓度。根据GO患者的疾病活动度分析CAS与泪液8-OHdG和MDA浓度之间的相关性。

结果

对照组中8-OHdG和MDA的水平分别为56.30±16.81 ng/mL和5.39±1.31 pmol/mg,非活动期GO患者分别为123.46±22.67 ng/mL和13.59±3.93 pmol/mg,活动期GO患者分别为215.14±35.61 ng/mL和22.52±4.63 pmol/mg。与对照组相比,非活动期和活动期GO患者的8-OHdG和MDA平均浓度更高(均P<0.001)。此外,在活动期组中,泪液中8-OHdG和MDA的浓度高于非活动期组(均P<0.001)。活动期GO组中8-OHdG水平(r=0.676,P<0.001)和MDA水平(r=0.506,P=0.002)与CAS相关。

结论

GO患者泪液中8-OHdG和MDA的浓度升高,尤其是活动期患者。在活动期GO患者中,CAS与泪液8-OHdG和MDA水平显著相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8da/6249971/069d8182c80d/12886_2018_969_Fig1_HTML.jpg

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