Semashko Vadim V, Pudovkin Maksim S, Cefalas Alkiviadis-Constantinos, Zelenikhin Pavel V, Gavriil Vassilios E, Nizamutdinov Alexei S, Kollia Zoe, Ferraro Angelo, Sarantopoulou Evangelia
Institute of Physics, Kazan Federal University, 18 Kremljovskaja str, Kazan, 420008, Russia.
National Hellenic Research Foundation, Theoretical and Physical Chemistry Institute, 48 Vassileos Constantinou Avenue, 11635, Athens, Greece.
Nanoscale Res Lett. 2018 Nov 21;13(1):370. doi: 10.1186/s11671-018-2775-z.
Localised extracellular interactions between nanoparticles and transmembrane signal receptors may well activate cancer cell growth. Herein, tiny LaF and PrF nanoparticles in DMEM+FBS suspensions stimulated tumour cell growth in three different human cell lines (A549, SW837 and MCF7). Size distribution of nanoparticles, activation of AKT and ERK signalling pathways and viability tests pointed to mechanical stimulation of ligand adhesion binding sites of integrins and EGFR via a synergistic action of an ensemble of tiny size nanoparticles (< 10 nm). While tiny size nanoparticles may be well associated with the activation of EGFR, integrin interplay with nanoparticles remains a multifaceted issue. A theoretical motif shows that, within the requisite pN force scale, each ligand adhesion binding site can be activated by a tiny size dielectric nanoparticle via electrical dipole interaction. The size of the active nanoparticle stayed specified by the amount of the surface charges on the ligand adhesion binding site and the nanoparticle, and also by the separating distance between them. The polar component of the electrical dipole force remained inversely proportional to the second power of nanoparticle's size, evincing that only tiny size dielectric nanoparticles might stimulate cancer cell growth via electrical dipole interactions. The work contributes towards recognising different cytoskeletal stressing modes of cancer cells.
纳米颗粒与跨膜信号受体之间的局部细胞外相互作用很可能会激活癌细胞生长。在此，DMEM+FBS悬浮液中的微小LaF和PrF纳米颗粒刺激了三种不同人类细胞系（A549、SW837和MCF7）中的肿瘤细胞生长。纳米颗粒的尺寸分布、AKT和ERK信号通路的激活以及活力测试表明，通过一组微小尺寸（<10 nm）纳米颗粒的协同作用，对整合素和表皮生长因子受体（EGFR）的配体粘附结合位点进行了机械刺激。虽然微小尺寸纳米颗粒可能与EGFR的激活密切相关，但整合素与纳米颗粒的相互作用仍然是一个多方面的问题。一个理论模型表明，在所需的皮牛力尺度内，每个配体粘附结合位点可通过微小尺寸的介电纳米颗粒经电偶极相互作用而被激活。活性纳米颗粒的尺寸由配体粘附结合位点和纳米颗粒上的表面电荷量以及它们之间的分隔距离确定。电偶极力的极性分量与纳米颗粒尺寸的二次方成反比，表明只有微小尺寸的介电纳米颗粒可能通过电偶极相互作用刺激癌细胞生长。这项工作有助于认识癌细胞的不同细胞骨架应激模式。
