Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh.
Department of Biochemistry & Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh.
Biomed Pharmacother. 2019 Feb;110:74-84. doi: 10.1016/j.biopha.2018.11.033. Epub 2018 Nov 19.
This research investigated the functional food effect of Leea macrophylla (Roxb.) ex Hornem root extract on pancreatic necrosis in Streptozotocin-induced type-2 diabetes. Prior to animal intervention, Leea macrophylla root extract (LMR) was subjected to GC-MS analysis. Across a three-week intervention of fructose-fed albino model with LMR50, LMR100 and LMR200, the fluid & food intake, body weight changes, weekly blood glucose concentrations and oral glucose tolerance (OGT) were recorded. The animals were sacrificed after intervention and serum was analyzed for insulin, ALT, AST, LDH, CK-MB, creatinine, uric acid and lipid profile and liver section was used for glycogen estimation. Changes of pancreas and kidney architecture were evaluated by histopathology. Relative mRNA for superoxide dismutase 1 (SOD1), glutathione peroxidase (GPx) and catalase (CAT) were quantitated using assay kits. Results showed that fluid and food intake, weekly blood glucose level, ALT, AST, LDH, CK-MB level were significantly (p < 0.05) decreased in LMR50 group. Conversely, the glucose tolerance ability, liver glycogen level, serum insulin, organ weight and pancreatic morphology were improved significantly in this group. Diameter of islet of Langerhans (μm), area occupied by β-cell/ islet of Langerhans (μm) and number of β-cells/islet of Langerhans were amazingly improved to the NC animals. Expressions of mRNA for SOD1 and CAT from liver tissue have been found to be increased multifold while GPx was remained unchanged. The data suggests that L. macrophylla root extract could be very potential as functional food to modulate pancreatic action.
本研究调查了罗比李根提取物对链脲佐菌素诱导的 2 型糖尿病胰腺坏死的功能性食品作用。在动物干预之前，对罗比李根提取物（LMR）进行了 GC-MS 分析。在为期三周的干预中，给果糖喂养的白化模型喂食 LMR50、LMR100 和 LMR200，记录液体和食物摄入量、体重变化、每周血糖浓度和口服葡萄糖耐量（OGT）。干预后，动物被处死，分析血清中的胰岛素、ALT、AST、LDH、CK-MB、肌酐、尿酸和脂质谱，并使用肝段估计糖原。通过组织病理学评估胰腺和肾脏结构的变化。使用试剂盒定量测定超氧化物歧化酶 1（SOD1）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的相对 mRNA。结果表明，LMR50 组的液体和食物摄入量、每周血糖水平、ALT、AST、LDH、CK-MB 水平均显著降低（p<0.05）。相反，该组的葡萄糖耐量能力、肝糖原水平、血清胰岛素、器官重量和胰腺形态均显著改善。胰岛 Langerhans 细胞的直径（μm）、β-细胞/胰岛 Langerhans 细胞的面积（μm）和β-细胞/胰岛 Langerhans 细胞的数量惊人地恢复到 NC 动物的水平。肝组织中 SOD1 和 CAT 的 mRNA 表达倍数增加，而 GPx 保持不变。数据表明，罗比李根提取物可能是一种很有潜力的功能性食品，可调节胰腺功能。
