Kyoto University, Research and Educational Unit of Leaders for Integrated Medical System, Center for, Japan.
Beijing Institute of Technology, Human Brain Research Laboratory, Intelligent Robotics Institute, Be, China.
J Biomed Opt. 2018 Nov;24(3):1-11. doi: 10.1117/1.JBO.24.3.031014.
Infrared thermal imaging of brain temperature changes is useful for evaluating cortical activity and disease states, such as stroke. However, the changes depend on a balance between changes in heat generation from metabolism and in heat convection related to blood flow. To discriminate between these effects and gain a clearer understanding of neurovascular metabolic coupling, brain temperature imaging must be improved to measure temperature and blood flow simultaneously. We develop an imaging technique that shows a two-dimensional (2-D) distribution of absolute brain temperature and relative cerebral blood flow changes in anesthetized rats by combining infrared thermal imaging with laser speckle imaging. The changes in brain metabolism and cerebral blood flow are achieved using two different anesthetics (isoflurane and α-chloralose) to evaluate our system. Isoflurane increased cerebral blood flow but decreased metabolism, whereas α-chloralose decreased both parameters. This technique enables simultaneous visualization of brain surface changes in temperature and cerebral blood flow in the same regions. This imaging system will permit further study of neurovascular metabolic coupling in normal and diseased brains.
脑温变化的红外热成像对于评估皮质活动和疾病状态（如中风）非常有用。然而，这些变化取决于代谢产生的热量变化与血流相关的热对流之间的平衡。为了区分这些影响，并更清楚地了解神经血管代谢偶联，必须改进脑温成像以同时测量温度和血流。我们开发了一种成像技术，通过将红外热成像与激光散斑成像相结合，显示麻醉大鼠大脑温度和相对脑血流变化的二维（2-D）分布。使用两种不同的麻醉剂（异氟烷和α-氯醛）来改变脑代谢和脑血流，以评估我们的系统。异氟烷增加了脑血流但降低了代谢，而α-氯醛则降低了这两个参数。该技术能够同时可视化同一区域的大脑表面温度和脑血流变化。该成像系统将允许进一步研究正常和患病大脑中的神经血管代谢偶联。
