Departamento de Patologia e Medicina Legal, Programa de Pós-graduação em Microbiologia Médica, Universidade Federal do Ceará, 50430-275 Fortaleza - CE, Brazil; Faculdade Paulo Picanço, 60135-218, Fortaleza-CE, Brazil.
Departamento de Ciências Naturais, Matemática e Estatística, Universidade Federal Rural do Semi-Árido, 59625-900 Mossoró - RN, Brazil.
Photodiagnosis Photodyn Ther. 2019 Mar;25:111-118. doi: 10.1016/j.pdpdt.2018.11.016. Epub 2018 Nov 20.
Rose Bengal@α-cyclodextrin (RB@α-CD) microparticles (μPs) were prepared and the RB inclusion in α-CD was experimentally demonstrated through infrared, UV-VIS absorption spectroscopy and cyclic voltammetry. The RB inclusion in α-CD was theoretically investigated using classical molecular mechanics calculations, the simulation results showing that RB can be included in both the narrow and wide apertures of the α-cyclodextrin ring with configurations exhibiting average binding energies of about 27 kcal mol. The prepared RB@α-CD microparticles were characterized through Scanning Electron Microscopy (SEM) and it was demonstrated that they are highly efficient in the photodynamic therapy against a Streptococcus mutans (the main bacteria of cariogenic dental plaque) suspension, as a concentration of RB@α-CD μPs 10 times smaller than the usual concentration of pure RB is still capable to produce significant antibacterial activity.
玫瑰红 Bengal@α-环糊精（RB@α-CD）微球（μPs）被制备，RB 与 α-CD 的包合通过红外、紫外可见吸收光谱和循环伏安法进行了实验验证。RB 与 α-CD 的包合通过经典分子力学计算进行了理论研究，模拟结果表明 RB 可以被包含在 α-环糊精环的窄和宽孔中，具有约 27 kcal/mol 的平均结合能的结构。通过扫描电子显微镜（SEM）对制备的 RB@α-CD 微球进行了表征，结果表明它们在抗变形链球菌（致龋牙菌斑的主要细菌）悬浮液的光动力疗法中非常有效，因为 RB@α-CD μPs 的浓度比纯 RB 的常用浓度小 10 倍，仍能产生显著的抗菌活性。
