Ono H, Iwasaki M, Sakamoto N, Mizuno S
Department of Agricultural Chemistry, Tohoku University, Sendai, Japan.
Gene. 1988 Jun 15;66(1):77-85. doi: 10.1016/0378-1119(88)90226-0.
A cDNA clone, pLOA0511, was isolated from the cDNA library prepared from the left ovaries of one- to three-day-old chickens. The transcript corresponding to this cDNA clone is approx. 1.9 kb in length and is present in steroidogenic tissues (ovary, testis and adrenal gland) but is undetectable in non-steroidogenic tissues. Relative abundance of the transcript in the ovary and testis is high and developmentally regulated but is much lower in the adrenal gland. Relative levels of this transcript in the developing left ovary and the regressing right ovary of the one- to three-day-old chicken are similar. The nucleotide sequence of this cDNA clone shows significant homology to some mammalian cytochromes P-450. Out of the 508 amino acids (aa) coded, 244 and 243 aa residues are identical with those of the bovine and human cytochrome P-450c17 (steroid 17 alpha-hydroxylase/17,20 lyase), respectively. There are three highly conserved regions among the bovine, human and chicken sequences, one of which is unique to P-450c17. These data suggest strongly that this cDNA corresponds to the mRNA of a chicken cytochrome P-450c17.
从1至3日龄鸡的左侧卵巢制备的cDNA文库中分离出一个cDNA克隆pLOA0511。与该cDNA克隆对应的转录本长度约为1.9 kb，存在于类固醇生成组织（卵巢、睾丸和肾上腺）中，但在非类固醇生成组织中检测不到。该转录本在卵巢和睾丸中的相对丰度较高且受发育调控，但在肾上腺中则低得多。1至3日龄鸡发育中的左侧卵巢和退化中的右侧卵巢中该转录本的相对水平相似。该cDNA克隆的核苷酸序列与一些哺乳动物细胞色素P - 450具有显著同源性。在编码的508个氨基酸（aa）中，分别有244个和243个氨基酸残基与牛和人细胞色素P - 450c17（类固醇17α - 羟化酶/17,20裂解酶）的氨基酸残基相同。在牛、人和鸡的序列中有三个高度保守的区域，其中一个是P - 450c17特有的。这些数据强烈表明该cDNA对应于鸡细胞色素P - 450c17的mRNA。
