Medical University of Białystok and Private Dental Office in Białystok, ul. Rzemieślnicza 37, Białystok 15-773, Poland.
Otolaryngology Department, Medical University of Białystok, M. Skłodowskiej-Curie 24 A, 15-276 Białystok, Poland.
Oxid Med Cell Longev. 2018 Oct 24;2018:7083486. doi: 10.1155/2018/7083486. eCollection 2018.
Oxidative stress takes part in the pathomechanisms of many diseases, including oral disorders. The imbalance between oxidative and antioxidative processes may lead to periodontitis, osteitis, or oral cancers. Furthermore, many chemotherapeutics, e.g., metronidazole (MTZ), may also cause toxic reactions and affect oxidative reactions. The research focused on MTZ influence on oxidative destruction in the parotid and submandibular gland tissue in animal experimental model. Therefore, the concentrations of enzymatic and nonenzymatic markers of oxidative stress were measured in these two rat glands in the control and experimental MTZ-treated groups. The material for analysis included parotid and submandibular glands of male Wistar rats, which were treated with metronidazole for 7 days by gastric tube in a dose of 100 mg/kg b.w. On day 8, the material was obtained and frozen in temp. -80°C. Then, the following seven enzymatic and nonenzymatic parameters were measured: GPx, TOS, TAS, SOD, LPO, CAT, and GSH. The data were analysed using Statistica 10.0. Metronidazole treatment in the experimental model showed an increase in LPO, TOS, and TOS/TAS and a decrease in CAT, SOD, GPx, and TAS. The conclusions of this research were made. Metronidazole treatment in a dose of 100 mg/kg b.w. caused imbalance between oxidative and antioxidative reactions in the rat parotid and submandibular glands. An increase was observed in LPO, TOS, and TOS/TAS in both glands exposed to metronidazole. Decreased activity of CAT, SOD, GPx, and TAS was noted, which indicates attenuation of the gland antioxidative protective barrier.
氧化应激参与许多疾病的病理机制，包括口腔疾病。氧化和抗氧化过程之间的失衡可能导致牙周炎、骨炎或口腔癌。此外，许多化疗药物，如甲硝唑（MTZ），也可能引起毒性反应并影响氧化反应。本研究聚焦于 MTZ 对动物实验模型中腮腺和颌下腺组织氧化破坏的影响。因此，在对照组和 MTZ 处理组的这两种大鼠腺体中测量了氧化应激的酶和非酶标记物的浓度。分析材料包括雄性 Wistar 大鼠的腮腺和颌下腺，这些大鼠通过胃管用 100mg/kg bw 的剂量接受 MTZ 治疗 7 天。第 8 天，获得并在 -80°C 温度下冷冻材料。然后，测量了以下七种酶和非酶参数：GPx、TOS、TAS、SOD、LPO、CAT 和 GSH。使用 Statistica 10.0 分析数据。实验模型中的 MTZ 处理显示 LPO、TOS 和 TOS/TAS 增加，CAT、SOD、GPx 和 TAS 减少。得出以下结论。MTZ 以 100mg/kg bw 的剂量处理导致大鼠腮腺和颌下腺的氧化和抗氧化反应失衡。暴露于 MTZ 的两种腺体中观察到 LPO、TOS 和 TOS/TAS 增加。CAT、SOD、GPx 和 TAS 的活性降低，表明腺体抗氧化保护屏障减弱。
