Smit V T, Boot A J, Smits A M, Fleuren G J, Cornelisse C J, Bos J L
Department of Pathology, State University of Leiden, The Netherlands.
Nucleic Acids Res. 1988 Aug 25;16(16):7773-82. doi: 10.1093/nar/16.16.7773.
DNAs from human pancreatic adenocarcinomas were analyzed for the presence of mutations in codons 12, 13 and 61 of the NRAS, KRAS and HRAS gene. Formalin-fixed and paraffin-embedded tissue was used directly in an in vitro amplification reaction to expand the relevant RAS sequences. The mutations were detected by selective hybridization using mutation-specific synthetic oligonucleotides. In 28 of the 30 patients we found a mutation in codon 12 of the KRAS gene. This result confirms the findings of Almoguera et al. [Cell 53 (1988) 549-554] that KRAS mutations occur frequently in adenocarcinomas of the exocrine pancreas. The mutations are predominantly G-T transversions, in contrast to the KRAS mutations in colon tumors which are mainly G-A transitions. Furthermore, in a portion of the tumors the mutation appears to be homozygous.
对来自人类胰腺腺癌的DNA进行分析，以检测NRAS、KRAS和HRAS基因第12、13和61密码子处是否存在突变。福尔马林固定石蜡包埋组织直接用于体外扩增反应，以扩增相关的RAS序列。使用突变特异性合成寡核苷酸通过选择性杂交检测突变。在30例患者中的28例中，我们发现KRAS基因第12密码子处存在突变。这一结果证实了阿尔莫格拉等人[《细胞》53卷（1988年）549 - 554页]的发现，即KRAS突变在外分泌性胰腺腺癌中频繁发生。这些突变主要是G - T颠换，这与结肠肿瘤中的KRAS突变主要是G - A转换形成对比。此外，在部分肿瘤中，突变似乎是纯合的。
