Department of Restorative Dentistry, Araraquara School of Dentistry, São Paulo State University-UNESP, Araraquara, São Paulo, Brazil.
Department of Restorative Dentistry, Araraquara School of Dentistry, São Paulo State University-UNESP, Araraquara, São Paulo, Brazil.
J Endod. 2018 Dec;44(12):1812-1816. doi: 10.1016/j.joen.2018.09.009.
The aim of this study was to assess the influence of antibiotic formulations (tablet/capsule [TC] or United States Pharmacopeia [USP]-grade antibiotics) and vehicles (water [HO] or macrogol + propylene glycol [MP]) on the cytotoxicity and pH of triple antibiotic pastes (TAPs).
L929 fibroblasts were exposed to TAPs prepared with TC or USP-grade antibiotics mixed with HO or MP for 72 hours. Each isolated antibiotic with each vehicle, each isolated vehicle, and the culture medium were used as controls. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and neutral red assays. The pH was measured after 3 and 8 hours of immersion of the pastes in water. Data were analyzed using analysis of variance, the Bonferroni or Tukey posttests (α = 0.05), and the Pearson correlation test (α = 0.05).
The pastes prepared with TC were less cytotoxic than pastes prepared with USP-grade antibiotics (P < .05), and pastes with the MP vehicle were less cytotoxic than pastes with HO (P < .05). TC TAP + MP showed the lowest cytotoxicity, whereas USP-grade TAP + HO showed the highest cytotoxicity (P < .05). All TAPs showed a pH ranging from 4.64-5.20. Irrespective of the vehicle, USP-grade TAP showed a lower pH than TC TAP (P < .05). TAPs with HO had a lower pH than TAPs with MP (P < .05).
The vehicle and the antibiotic formulations influenced the cytotoxicity and pH of TAP. The pastes prepared with TC and MP were less acidic and less cytotoxic than the type prepared with USP-grade antibiotics and HO.
本研究旨在评估抗生素制剂（片剂/胶囊[TC]或美国药典[USP]级抗生素）和载体（水[HO]或聚乙二醇+丙二醇[MP]）对三联抗生素糊剂（TAP）的细胞毒性和 pH 的影响。
将 L929 成纤维细胞暴露于 TC 或 USP 级抗生素与 HO 或 MP 混合制备的 TAP 中 72 小时。每种分离的抗生素与每种载体、每种分离的载体和培养基均用作对照。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物和中性红测定评估细胞毒性。将糊剂在水中浸泡 3 和 8 小时后测量 pH。使用方差分析、Bonferroni 或 Tukey 后检验（α=0.05）以及 Pearson 相关检验（α=0.05）分析数据。
与 USP 级抗生素制备的糊剂相比，TC 制备的糊剂的细胞毒性较低（P<0.05），并且 MP 载体的糊剂的细胞毒性低于 HO（P<0.05）。TC TAP+MP 显示出最低的细胞毒性，而 USP 级 TAP+HO 显示出最高的细胞毒性（P<0.05）。所有 TAP 均显示 pH 值在 4.64-5.20 之间。无论载体如何，USP 级 TAP 的 pH 值均低于 TC TAP（P<0.05）。HO 中的 TAP 的 pH 值低于 MP 中的 TAP（P<0.05）。
载体和抗生素制剂影响 TAP 的细胞毒性和 pH 值。与 USP 级抗生素和 HO 制备的糊剂相比，TC 和 MP 制备的糊剂的酸性较低，细胞毒性较小。
