Wang S S, Chiu C C, Tseing J M, Wang S R
Department of Pediatrics, Kaohsiung Medical College, Taiwan, ROC.
Asian Pac J Allergy Immunol. 1988 Jun;6(1):3-6.
Microtiter plates have been popularly used for lymphocyte culture, but the influence of culture plates from different sources has not been investigated. In this study, the degree of mitogen-induced cell proliferation was investigated using six different brands of flat-bottomed plates. Lymphocytes from twelve normal donors were cultured for 96 hours with several mitogens including PHA, Con A and PWM. Spontaneous cell proliferation was slow and it did not differ significantly among the different plates. However, mitogen-induced cell proliferation showed a wide variation among the six types of plates used. The importance of selecting certain kinds of plates for specific purposes is emphasized.
微量滴定板已广泛用于淋巴细胞培养，但不同来源的培养板的影响尚未得到研究。在本研究中，使用六个不同品牌的平底培养板研究了丝裂原诱导的细胞增殖程度。来自12名正常供体的淋巴细胞与包括PHA、Con A和PWM在内的几种丝裂原一起培养96小时。自发细胞增殖缓慢，不同培养板之间没有显著差异。然而，丝裂原诱导的细胞增殖在所使用的六种培养板之间表现出很大差异。强调了为特定目的选择某些类型培养板的重要性。
