从黑树莓中分离出的菌株的特性及其对BALB/c小鼠肠道微生物群的影响。

Characterization of strains isolated from black raspberry and their effect on BALB/c mice gut microbiota.

作者信息

Choi Hye Ran, Chung Yi Hyung, Yuk Hyun-Gyun, Lee Hyunki, Jang Han Su, Kim Yosum, Shin Daekeun

机构信息

Berry & Biofood Research Institute, Gochang, Republic of Korea.

Jeonbuk Institute for Food-Bioindustry, Jeonju, Republic of Korea.

出版信息

Food Sci Biotechnol. 2018 Jun 26;27(6):1747-1754. doi: 10.1007/s10068-018-0420-3. eCollection 2018 Dec.

DOI:10.1007/s10068-018-0420-3

PMID:30483439

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6233400/

Abstract

The objective of this study was to evaluate probiotic effects of two strains (GBL16 and 17) isolated from black raspberry. Results revealed that the number of GBL16 was gradually decreased as bile salt concentration was increased from 0.3 to 1%. However, GBL17 did not show any difference when GBL17 was applied to 1% bile salt, and it indicates that GBL17 is more tolerant to bile salt than GBL16. GBL17 exhibited higher heat resistance and adhesion ability to Caco-2 cells than GBL16. Regarding gut microbiome, no significant change in the number of total bacteria in intestines of mice after treatment with GBLs was determined. However, the combination of GBL16 and GBL17 significantly increased the number of total bacteria in intestines of mice after they were orally administered. Therefore, the results suggest that both GBL16 and 17 strains could be one of major probiotics that can improve human gut health.

摘要

本研究的目的是评估从黑树莓中分离出的两株菌株（GBL16和GBL17）的益生菌效果。结果显示，随着胆盐浓度从0.3%增加到1%，GBL16的数量逐渐减少。然而，当GBL17应用于1%胆盐时，未显示出任何差异，这表明GBL17比GBL16对胆盐的耐受性更强。GBL17比GBL16表现出更高的耐热性和对Caco-2细胞的粘附能力。关于肠道微生物群，在用GBL处理后，小鼠肠道中总细菌数量没有显著变化。然而，口服GBL16和GBL17的组合后，显著增加了小鼠肠道中总细菌的数量。因此，结果表明GBL16和17菌株都可能是改善人类肠道健康的主要益生菌之一。