Louis J M, McFarland V W, May P, Mora P T
Division of Cancer Biology and Diagnosis, National Cancer Institute, Bethesda, MD 20892.
Biochim Biophys Acta. 1988 Sep 7;950(3):395-402. doi: 10.1016/0167-4781(88)90136-4.
The phosphoprotein p53 has been investigated mainly because of its relationship with tumorigenic transformation. In this communication, we report that, during the embryonal development of mouse and chicken, there is a decline in the steady-state levels of the p53 protein and an equal decline in p53 mRNA. During the development of the chicken, the relative rates of p53 transcription appear to be constant. p53 mRNA is relatively stable (half-life greater than 12 h) in both chicken and mouse embryos. We conclude that (i) the down-modulation of p53 mRNA (and of protein) during embryonal development has been well conserved during the evolution of the vertebrate, implying that the p53 protein may have a function in embryonal development; and (ii) the mechanism of control is apparently mainly on a post-transcriptional level.
磷蛋白p53一直是研究的重点，主要是因为它与肿瘤转化有关。在本报告中，我们发现在小鼠和鸡的胚胎发育过程中，p53蛋白的稳态水平下降，p53 mRNA也有同等程度的下降。在鸡的发育过程中，p53转录的相对速率似乎保持恒定。p53 mRNA在鸡和小鼠胚胎中都相对稳定（半衰期大于12小时）。我们得出以下结论：（i）在脊椎动物进化过程中，胚胎发育期间p53 mRNA（和蛋白质）的下调现象保存良好，这意味着p53蛋白可能在胚胎发育中发挥作用；（ii）调控机制显然主要在转录后水平。
