Wang Xiaofeng, Zhao Zheng, Zhang Wei, Wang Yi
Department of Cardiology, Cangzhou Central Hospital, Cangzhou, China.
Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou, China.
J Cell Biochem. 2019 May;120(5):8214-8221. doi: 10.1002/jcb.28103. Epub 2018 Nov 28.
Long noncoding RNAs (lncRNAs) have been showed to play a crucial role in pathogenesis and development of cardiovascular diseases. Our study aimed to study the expression and functional role of lncRNA LINC00968 in the development of coronary artery disease (CAD). We showed that the LINC00968 expression level was upregulated in the CAD tissues compared with normal arterial tissues. In addition, we showed that the expression level of LINC00968 was upregulated by oxidized low-density lipoprotein (oxLDL) treatment in endothelial cell. Ectopic expression of LINC00968 regulated the proliferation and migration of endothelial cell. Moreover, we showed that overexpression of LINC00968 inhibited miR-9-3p expression in an endothelial cell. Furthermore, we demonstrated that the miR-9-3p expression was downregulated in the CAD samples compared with normal arterial tissues and the expression level of miR-9-3p was downregulated by oxLDL treatment in endothelial cell. Finally, we showed that ectopic expression of LINC00968 promoted endothelial cell proliferation and migration partly through regulating miR-9-3p expression. These results suggested that LINC00968 plays a crucial role in the progression of the CAD.
长链非编码RNA（lncRNAs）已被证明在心血管疾病的发病机制和发展过程中起关键作用。我们的研究旨在探讨lncRNA LINC00968在冠状动脉疾病（CAD）发展中的表达及功能作用。我们发现，与正常动脉组织相比，CAD组织中LINC00968的表达水平上调。此外，我们还发现，氧化型低密度脂蛋白（oxLDL）处理可使内皮细胞中LINC00968的表达水平上调。LINC00968的异位表达可调节内皮细胞的增殖和迁移。此外，我们发现LINC00968的过表达可抑制内皮细胞中miR-9-3p的表达。此外，我们还证明，与正常动脉组织相比，CAD样本中miR-9-3p的表达下调，且oxLDL处理可使内皮细胞中miR-9-3p的表达水平下调。最后，我们发现LINC00968的异位表达部分通过调节miR-9-3p的表达促进内皮细胞的增殖和迁移。这些结果表明，LINC00968在CAD的进展中起关键作用。
