Grupo de Investigación en Ingeniería Biomédica, Vicerrectoría de Investigaciones, Universidad Manuela Beltrán, Avenida Circunvalar No. 60-00, Bogotá 1101, Colombia.
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 76001 Zlín, Czech Republic.
Molecules. 2018 Nov 28;23(12):3109. doi: 10.3390/molecules23123109.
Bio-artificial polymeric systems are a new class of polymeric constituents based on blends of synthetic and natural polymers, designed with the purpose of producing new materials that exhibit enhanced properties with respect to the individual components. In this frame, a combination of polyvinyl alcohol (PVA) and chitosan, blended with a widely used antibiotic, sodium ampicillin, has been developed showing a moderate behavior in terms of antibacterial properties. Thus, aqueous solutions of PVA at 1 wt.% were mixed with acid solutions of chitosan at 1 wt.%, followed by adding ampicillin ranging from 0.3 to 1.0 wt.% related to the total amount of the polymers. The prepared bio-artificial polymeric system was characterized by FTIR, SEM, DSC, contact angle measurements, antibacterial activity against and and antibiotic release studies. The statistical significance of the antibacterial activity was determined using a multifactorial analysis of variance with ρ < 0.05 (ANOVA). The characterization techniques did not show alterations in the ampicillin structure and the interactions with polymers were limited to intermolecular forces. Therefore, the antibiotic was efficiently released from the matrix and its antibacterial activity was preserved. The system disclosed moderate antibacterial activity against bacterial strains without adding a high antibiotic concentration. The findings of this study suggest that the system may be effective against healthcare-associated infections, a promising view in the design of novel antimicrobial biomaterials potentially suitable for tissue engineering applications.
生物人工聚合体系是一类新型聚合材料，由合成聚合物和天然聚合物混合而成，旨在制备具有增强性能的新材料，这些性能优于单个组分。在这一框架内，将聚乙烯醇（PVA）和壳聚糖与一种广泛使用的抗生素氨苄西林钠混合，开发出了一种具有适度抗菌性能的材料。具体方法是：将 1wt%的 PVA 水溶液与 1wt%的壳聚糖酸溶液混合，然后加入 0.3-1.0wt%（相对于聚合物总量）的氨苄西林。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、差示扫描量热法（DSC）、接触角测量、对 和 的抗菌活性以及抗生素释放研究对制备的生物人工聚合体系进行了表征。使用ρ<0.05（方差分析（ANOVA））的多因素方差分析确定抗菌活性的统计学意义。表征技术未显示出氨苄西林结构的变化，并且聚合物之间的相互作用仅限于分子间力。因此，抗生素可以从基质中有效释放出来，并且其抗菌活性得以保留。该系统对细菌菌株表现出适度的抗菌活性，而无需添加高浓度的抗生素。本研究的结果表明，该系统可能对与医疗保健相关的感染有效，这为设计新型抗菌生物材料提供了有希望的观点，这些材料可能适用于组织工程应用。
