Department of Cellular & Integrative Physiology, Indiana University School of Medicine, 635 Barnhill Drive, MS 385, Indianapolis, IN, 46202, USA.
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
J Cardiovasc Transl Res. 2019 Jun;12(3):211-220. doi: 10.1007/s12265-018-9849-2. Epub 2018 Nov 28.
Intravascular photoacoustic-ultrasound (IVPA-US) imaging and near-infrared spectroscopy-intravascular ultrasound (NIRS-IVUS) are two hybrid modalities that detect arterial lipid, with comparison necessary to understand the relative advantages of each. We performed in vivo and ex vivo IVPA-US imaging of the iliac arteries of Ossabaw swine with metabolic syndrome (MetS) and lean swine to investigate sensitivity for early-stage atherosclerosis. We repeated imaging ex vivo with NIRS-IVUS for comparison to IVPA-US and histology. Both modalities showed significantly greater lipid in MetS vs. lean swine, but only IVPA-US localized the lipid as perivascular. To investigate late-stage atherosclerosis, we performed ex vivo IVPA-US imaging of a human coronary artery with comparison to NIRS-IVUS and histology. Two advanced fibroatheromas were identified, with agreement between IVPA-measured lipid area and NIRS-derived lipid content. As confirmed histologically, IVPA-US has sensitivity to detect lipid content similar to NIRS-IVUS and provides additional depth resolution, enabling quantification and localization of lipid cores within plaques.
血管内光声-超声（IVPA-US）成像和近红外光谱-血管内超声（NIRS-IVUS）是两种混合模式，用于检测动脉脂质，需要进行比较以了解每种模式的相对优势。我们对代谢综合征（MetS）和瘦猪的 Ossabaw 猪的髂动脉进行了体内和离体 IVPA-US 成像，以研究早期动脉粥样硬化的敏感性。我们用 NIRS-IVUS 重复进行了离体成像，以与 IVPA-US 和组织学进行比较。两种模式都显示出 MetS 猪的脂质明显多于瘦猪，但只有 IVPA-US 将脂质定位于血管周围。为了研究晚期动脉粥样硬化，我们对人类冠状动脉进行了离体 IVPA-US 成像，并与 NIRS-IVUS 和组织学进行了比较。发现了两个高级纤维粥样瘤，IVPA 测量的脂质面积与 NIRS 衍生的脂质含量之间存在一致性。正如组织学所证实的，IVPA-US 具有检测脂质含量的敏感性，与 NIRS-IVUS 相似，并提供了额外的深度分辨率，能够定量和定位斑块内的脂质核心。
