31P核磁共振研究某些氯酚对大肠杆菌和一种五氯酚降解菌的影响。
31P nuclear magnetic resonance studies of effects of some chlorophenols on Escherichia coli and a pentachlorophenol-degrading bacterium.
作者信息
Steiert J G, Thoma W J, Ugurbil K, Crawford R L
机构信息
Gray Freshwater Biological Institute, University of Minnesota, Navarre 55392.
出版信息
J Bacteriol. 1988 Oct;170(10):4954-7. doi: 10.1128/jb.170.10.4954-4957.1988.
Abstract
A Flavobacterium sp. that mineralizes pentachlorophenol degrades some, but not all, of the other chlorinated phenols. Whole-cell 31P nuclear magnetic resonance was used to compare and observe transmembrane pH gradients and nucleotide pools in the Flavobacterium sp. and Escherichia coli after pentachlorophenol and 3,4,5-trichlorophenol were added to the cell suspensions. The data suggest that those chlorinated phenols which are not degraded by the Flavobacterium sp. may be resistant to degradation because they act as proton dissipators.
摘要
一种能使五氯苯酚矿化的黄杆菌属细菌可降解部分而非全部其他氯代酚。在细胞悬浮液中加入五氯苯酚和3,4,5-三氯苯酚后，利用全细胞31P核磁共振来比较和观察黄杆菌属细菌及大肠杆菌中的跨膜pH梯度和核苷酸库。数据表明，那些不能被黄杆菌属细菌降解的氯代酚可能因作为质子耗散剂而具有抗降解性。
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