Ando K, Natori S
Faculty of Pharmaceutical Sciences, University of Tokyo.
J Biochem. 1988 Apr;103(4):735-9. doi: 10.1093/oxfordjournals.jbchem.a122337.
The effect of sarcotoxin IIA, an antibacterial protein of Sarcophaga peregrina (flesh fly), on Escherichia coli was investigated. Sarcotoxin IIA was found to have a bacterial effect on growing bacteria, but little on non-growing bacteria. At a concentration of 25 micrograms/ml, it induced significant morphological change of growing E. coli cells. In its presence, growing cells became greatly elongated, and spheroplast-like bulges and projections appeared on their surface. A rough mutant strain of E. coli with a defect in the structure of lipopolysaccharide was more sensitive than the parent strain to sarcotoxin IIA. These results suggest that the main effect of sarcotoxin IIA is to inhibit cell wall synthesis, including septum formation.
研究了麻蝇（肉蝇）的抗菌蛋白麻蝇毒素IIA对大肠杆菌的作用。发现麻蝇毒素IIA对生长中的细菌有抗菌作用，但对非生长细菌作用很小。在浓度为25微克/毫升时，它会诱导生长中的大肠杆菌细胞发生显著的形态变化。在其存在下，生长中的细胞变得大大伸长，并且在其表面出现类似原生质球的凸起和突起。一种脂多糖结构有缺陷的大肠杆菌粗糙突变株比亲本菌株对麻蝇毒素IIA更敏感。这些结果表明，麻蝇毒素IIA的主要作用是抑制细胞壁合成，包括隔膜形成。
