Nakajima Y, Qu X M, Natori S
J Biol Chem. 1987 Feb 5;262(4):1665-9.
The direct interaction between phospholipids and sarcotoxin IA, a potent bactericidal protein of Sarcophaga peregrina, was studied using authentic sarcotoxin IA, its synthetic derivatives, and various liposomes. Results showed that sarcotoxin IA interacted with liposomes constituted from acidic phospholipids, resulting in the release of glucose trapped in these liposomes. The amidated carboxyl-terminal of this protein was found to be important for this interaction. Liposomes constituted from total phospholipids of Escherichia coli became less susceptible to sarcotoxin IA with an increase in their cholesterol content. Since bacterial membranes do not contain cholesterol, this finding may partly explain the selective toxicity of sarcotoxin I to bacteria.
利用纯正的蝇毒磷IA、其合成衍生物以及各种脂质体，研究了磷脂与蝇毒磷IA（一种强大的食蚜蝇杀菌蛋白）之间的直接相互作用。结果表明，蝇毒磷IA与由酸性磷脂构成的脂质体相互作用，导致包封在这些脂质体中的葡萄糖释放出来。发现该蛋白的酰胺化羧基末端对这种相互作用很重要。由大肠杆菌总磷脂构成的脂质体随着胆固醇含量的增加，对蝇毒磷IA的敏感性降低。由于细菌膜不含胆固醇，这一发现可能部分解释了蝇毒磷I对细菌的选择性毒性。
