Okada M, Natori S
Biochem J. 1985 Jul 15;229(2):453-8. doi: 10.1042/bj2290453.
When Escherichia coli was treated with sarcotoxin I, a potent bactericidal protein of Sarcophaga peregrina (fleshfly), K+ inside of the cells leaked out rapidly and the ATP pool of the cells rapidly decreased. These results suggested that the bactericidal effect of sarcotoxin I was due to its ionophore activity, and that it blocked the generation of ATP by inhibiting formation of the proton gradient essential for oxidative phosphorylation. This was confirmed by use of an uncA mutant, which was much less susceptible than the wild-type strain to sarcotoxin I under fixed ionic conditions.
当用麻蝇毒素I(一种棕尾别麻蝇的强效杀菌蛋白)处理大肠杆菌时,细胞内的K+迅速泄漏,细胞的ATP池迅速减少。这些结果表明,麻蝇毒素I的杀菌作用归因于其离子载体活性,并且它通过抑制氧化磷酸化所必需的质子梯度的形成来阻断ATP的生成。使用uncA突变体证实了这一点,在固定离子条件下,该突变体比野生型菌株对麻蝇毒素I的敏感性低得多。