Tamiya Hiroyuki, Mitani Akihisa, Saito Akira, Ishimori Taro, Saito Minako, Isago Hideaki, Jo Taisuke, Yamauchi Yasuhiro, Tanaka Goh, Nagase Takahide
The Department of Respiratory Medicine, The University of Tokyo Hospital, Tokyo, Japan.
The Department of Respiratory Medicine, The University of Tokyo Hospital, Tokyo, Japan
Anticancer Res. 2018 Dec;38(12):6707-6714. doi: 10.21873/anticanres.13039.
BACKGROUND/AIM: Cytological analysis for diagnosing malignant pleural effusion associated with lung adenocarcinoma (Ad-MPE) shows limited sensitivity and novel diagnostic biomarkers are needed. The aim of this study was to evaluate the profile of four microRNAs (miRNAs) in exosomes in Ad-MPE and benign (non-neoplastic) pleural effusion (BPE).
A total of 56 pleural effusion samples from patients with lung adenocarcinoma and benign diseases were collected. Exosomal miRNA expressions were evaluated using real-time quantitative reverse transcription polymerase chain reaction (RT-PCR).
The expression levels of miR-182 and miR-210 were significantly higher in the Ad-MPE than in the BPE samples. The receiver operating characteristics curve analyses of miR-182 and miR-210 for diagnosis of Ad-MPE yielded areas under the receiver operating characteristic curves of 0.87 and 0.81, respectively.
These miRNA signatures may have a diagnostic potential for differentiating Ad-MPE from BPE.
背景/目的：用于诊断与肺腺癌相关的恶性胸腔积液（Ad-MPE）的细胞学分析显示敏感性有限，因此需要新的诊断生物标志物。本研究的目的是评估Ad-MPE和良性（非肿瘤性）胸腔积液（BPE）中外泌体中四种微小RNA（miRNA）的特征。
共收集了56例来自肺腺癌患者和良性疾病患者的胸腔积液样本。使用实时定量逆转录聚合酶链反应（RT-PCR）评估外泌体miRNA的表达。
Ad-MPE中miR-182和miR-210的表达水平显著高于BPE样本。miR-182和miR-210用于诊断Ad-MPE的受试者工作特征曲线分析得出的受试者工作特征曲线下面积分别为0.87和0.81。
这些miRNA特征可能具有区分Ad-MPE和BPE的诊断潜力。
