Akindolire Muyiwa Ajoke, Kumar Ajay, Ateba Collins Njie
Department of Biological Sciences, School of Environmental and Health Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng Campus, South Africa.
Food Security and Safety Niche Area, Faculty of Agriculture, Science and Technology, North-West University, Mmabatho, Mafikeng 2735, South Africa.
Saudi J Biol Sci. 2018 Nov;25(7):1348-1355. doi: 10.1016/j.sjbs.2015.10.011. Epub 2015 Oct 23.
Food borne diseases are a major public health concern worldwide. is one of the potential food borne pathogens which causes nosocomial and community acquired infections. In the present study, 74 representative strains of isolated and characterized in previous study from different milk samples were subjected to random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) and enterobacterial repetitive intergenic consensus (ERIC)-PCR to generate fingerprints to determine the genetic relationships of the isolated strains. A total of 20 RAPD patterns were generated and the number of amplified fragments obtained ranged from 0 to 8 with molecular weight ranging from 250 to 2000 bp. A dendrogram based on fingerprinting pattern grouped isolates into twelve major clusters (I-XII). In the case of ERIC-PCR 9 banding patterns were obtained with amplicons ranging from 1 to 8 and band sizes ranging from 250 to 2000 bp. A total of four major clusters (I-IV) were observed in the dendrogram based on ERIC fingerprints. The discrete banding patterns obtained both from ERIC-PCR and RAPD-PCR showed remarkably the genetic diversity of . The findings of this study indicate that raw, bulk and pasteurized milk in the North-West Province was contaminated with toxigenic and multi-drug resistant strains. This emphasizes the need to implement appropriate control measures to reduce contamination as well as the spread of virulent strains to reduce the burden of disease in humans.
食源性疾病是全球主要的公共卫生问题。[病原体名称]是导致医院感染和社区获得性感染的潜在食源性病原体之一。在本研究中，对先前研究中从不同牛奶样本中分离并鉴定的74株[病原体名称]代表性菌株进行随机扩增多态性DNA（RAPD）聚合酶链反应（PCR）和肠杆菌重复基因间共识（ERIC）-PCR，以生成指纹图谱来确定分离菌株的遗传关系。共产生了20种RAPD图谱，获得的扩增片段数量从0到8不等，分子量范围为250至2000 bp。基于指纹图谱的聚类图将分离株分为十二个主要簇（I - XII）。在ERIC-PCR中，获得了9种条带模式，扩增子范围为1至8，条带大小范围为250至2000 bp。基于ERIC指纹图谱的聚类图中总共观察到四个主要簇（I - IV）。从ERIC-PCR和RAPD-PCR获得的离散条带模式显著显示了[病原体名称]的遗传多样性。本研究结果表明，西北省的生牛奶、散装牛奶和巴氏杀菌牛奶被产毒和耐多药的[病原体名称]菌株污染。这强调了实施适当控制措施以减少污染以及毒力[病原体名称]菌株传播的必要性，以减轻人类疾病负担。
