Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea.
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea.
J Biomed Mater Res B Appl Biomater. 2019 Aug;107(6):1937-1944. doi: 10.1002/jbm.b.34286. Epub 2018 Dec 3.
Fish bone extract (FBE) containing a trioligopeptide (FBP-KSA, Lys-Ser-Ala) isolated from Johnius belengerii could induce osteogenic activities on MC3T3-E1 pre-osteoblasts in our previous study. Regarding the osteogenic effect of FBE, in the present study, we fabricated the three-dimensional (3D) interconnected polycaprolactone (PCL)/FBE scaffolds for bone tissue regeneration. After fabrication of PCL scaffolds using 3D printing, FBE was coated on the surface of PCL scaffolds by self-assembly process. In the physical characteristic and mechanical property tests, the results demonstrated that the fabricated scaffolds have the strut diameter (between 340 and 345 μm), pore size (between 470 and 480 μm), porosity (between 50% and 55%), and tensile properties (Young's modulus: 9.18-9.42 MPa; max tensile strengths 82.3-87.4 MPa) were similar to those of PCL scaffold. In the cell proliferation and osteogenic assay, the results showed that PCL/FBE scaffolds could significantly induce cell proliferation, calcium deposition, and the expression of osteogenic phenotype markers such as alkaline phosphatase, osteopontin, osteocalcin, and bone morphogenetic protein-2 in the osteoblasts. These results suggest that FBE-coated PCL scaffolds are promising materials for use in biomedical application to promote bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1937-1944, 2019.
从褐牙鲆中分离得到的三肽（FBP-KSA，Lys-Ser-Ala）的鱼骨提取物（FBE）在我们之前的研究中可以诱导 MC3T3-E1 前成骨细胞的成骨活性。关于 FBE 的成骨作用，在本研究中，我们制备了用于骨组织再生的三维（3D）互穿聚己内酯（PCL）/FBE 支架。使用 3D 打印制备 PCL 支架后，通过自组装过程将 FBE 涂覆在 PCL 支架的表面上。在物理特性和机械性能测试中，结果表明所制备的支架具有支柱直径（340 至 345μm 之间）、孔径（470 至 480μm 之间）、孔隙率（50%至 55%之间）和拉伸性能（杨氏模量：9.18-9.42MPa；最大拉伸强度 82.3-87.4MPa）与 PCL 支架相似。在细胞增殖和成骨测定中，结果表明 PCL/FBE 支架可显著诱导细胞增殖、钙沉积以及碱性磷酸酶、骨桥蛋白、骨钙素和骨形态发生蛋白-2 等成骨表型标志物在成骨细胞中的表达。这些结果表明 FBE 涂覆的 PCL 支架是用于促进骨组织再生的生物医学应用的有前途的材料。©2018Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 107B：1937-1944，2019。
