Metcalf D, Moore J G
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7767-71. doi: 10.1073/pnas.85.20.7767.
A comparison was made of disease development in two lines of transgenic mice in which the granulocyte-macrophage colony-stimulating factor (GM-CSF) transgene was inserted in different chromosomal locations. Female-line mice (X chromosome insertion) had equivalent elevations of serum GM-CSF levels to those in male-line mice (autosomal insertion) but a shorter survival (median survival, 95 versus 145 days) and a significantly higher incidence of large inflammatory foci in skeletal muscle and gut congestion. Male-line transgenic mice had higher levels of cells in the peritoneal cavity and a higher frequency of spleen enlargement with excess erythropoiesis than female-line mice and uniquely developed fibrotic nodules in the abdominal and pleural cavities. The various diseases in GM-CSF transgenic mice are likely to have been induced by GM-CSF-stimulated products of macrophages, and in the two transgenic lines the macrophages exhibit characteristic differences in morphology and possibly functional activity.
对两系转基因小鼠的疾病发展情况进行了比较，在这两系小鼠中，粒细胞-巨噬细胞集落刺激因子（GM-CSF）转基因插入了不同的染色体位置。雌性系小鼠（X染色体插入）血清GM-CSF水平升高程度与雄性系小鼠（常染色体插入）相当，但生存期较短（中位生存期分别为95天和145天），骨骼肌中出现大的炎症病灶和肠道充血的发生率显著更高。雄性系转基因小鼠腹腔中的细胞水平较高，脾脏肿大且伴有红细胞生成过多的频率高于雌性系小鼠，并且在腹腔和胸腔中独特地形成了纤维化结节。GM-CSF转基因小鼠中的各种疾病可能是由GM-CSF刺激的巨噬细胞产物诱导的，在这两个转基因系中，巨噬细胞在形态以及可能的功能活性方面表现出特征性差异。
