Patwa Rahul, Soundararajan Narendren, Mulchandani Neha, Bhasney Siddharth M, Shah Manisha, Kumar Sachin, Kumar Amit, Katiyar Vimal
Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Department of Biosciences & Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Biopolymers. 2018 Nov;109(11):e23231. doi: 10.1002/bip.23231.
The article demonstrates the crystalline silk nano-discs (CSNs), with well-controlled morphology, which upon magnetization, yields magnetic crystalline silk nano-discs, making both prominent alternatives for replacing metal templates such as gold, silver, and so on in therapeutics and implants. The isolated β-sheet-rich discotic CSNs have ~50 nm diameter, high crystallinity (> 90%), and are insoluble but provide good dispersibility and stability in aqueous solutions. The melt blending-cum-electrospinning of functionalized CSN with poly(lactic acid) results in biocompatible nanofiber-based scaffolds having in vitro cell cytocompatibility with improved cell adhesion and proliferation. The assessment of release behavior of curcumin, a naturally occurring anticancer drug, shows sustained release over 25 days exhibiting effective cytotoxicity against human cervical cancer cells. Further, combined effect of curcumin and hyperthermia reduced the cell growth by ~63%. Alignment of CSN-derived magnetic nanoparticles due to effective fiber drawing process during electrospinning could improve cytocompatibility against BHK-21 cells, and therefore efficacy for cancer therapy.
该文章展示了具有形态可控的结晶丝纳米盘（CSNs），其在磁化后会产生磁性结晶丝纳米盘，这两者都是在治疗和植入物中替代金属模板（如金、银等）的突出选择。分离出的富含β-折叠的盘状CSNs直径约为50纳米，结晶度高（>90%），不溶于水，但在水溶液中具有良好的分散性和稳定性。功能化CSN与聚乳酸的熔融共混兼静电纺丝可得到具有生物相容性的基于纳米纤维的支架，其在体外具有细胞相容性，可改善细胞黏附和增殖。对天然抗癌药物姜黄素释放行为的评估表明，其在25天内持续释放，对人宫颈癌细胞表现出有效的细胞毒性。此外，姜黄素与热疗的联合作用使细胞生长减少了约63%。由于静电纺丝过程中有效的纤维拉伸过程，CSN衍生的磁性纳米颗粒的排列可提高对BHK-21细胞的细胞相容性，从而提高癌症治疗效果。
