Kurilenko Valeriya V, Romanenko Lyudmila A, Isaeva Marina P, Svetashev Vassilii I, Mikhailov Valery V
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, Russia, 690022.
Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia, 690041.
Antonie Van Leeuwenhoek. 2019 May;112(5):731-739. doi: 10.1007/s10482-018-1207-5. Epub 2018 Dec 5.
An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium Kr9-9 was isolated from a brown alga specimen collected near the Kuril Islands. Based on the 16S rRNA gene sequence analysis strain Kr9-9 was assigned to the genus Winogradskyella, and its close phylogenetic neighbors were found to be Winogradskyella damuponensis KCTC 23552, Winogradskyella sediminis LMG 28075, and Winogradskyella rapida CCUG 59098 showing high similarities of 98.1%, 97.5%, and 97.1%, respectively. It contained MK-6 as the predominant menaquinone and iso-C, anteiso-C, iso-C 3-OH followed by iso-C as the major fatty acids. Polar lipids included phosphatidylethanolamine, three unidentified aminolipids and an unidentified lipid. The DNA C+C content was 32.3 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Kr9-9 is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella algae sp. nov. is proposed. The type strain of the species is strain Kr9-9 (= KMM 8180 = KACC 19709).
从千岛群岛附近采集的褐藻标本中分离出一株需氧、革兰氏阴性、黄色色素沉着、无运动性的杆状细菌Kr9-9。基于16S rRNA基因序列分析，菌株Kr9-9被归入维诺格拉德斯基氏菌属，发现其亲缘关系较近的菌株为达蒙维诺格拉德斯基氏菌KCTC 23552、沉积物维诺格拉德斯基氏菌LMG 28075和快速维诺格拉德斯基氏菌CCUG 59098，相似性分别高达98.1%、97.5%和97.1%。它以MK-6作为主要的甲基萘醌，以异-C、anteiso-C、异-C 3-OH，其次是以异-C作为主要脂肪酸。极性脂质包括磷脂酰乙醇胺、三种未鉴定的氨基脂质和一种未鉴定的脂质。DNA的C+C含量为32.3摩尔%。基于系统发育分析和独特的表型特征，得出菌株Kr9-9代表维诺格拉德斯基氏菌属的一个新物种的结论，并为此提出维诺格拉德斯基氏海藻新种的名称。该物种的模式菌株为菌株Kr9-9（=KMM 8180=KACC 19709）。
