Heyuan Polytechnic, Heyuan, 517000, PR China.
Department of Biology, College of Science, Shantou University, Shantou 515063, PR China.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004577. Epub 2020 Dec 3.
A Gram-negative, aerobic, rod-shaped, non-flagellated and motile by gliding bacterium HL2-2, was isolated from the surface of the brown alga in China. The 16S rRNA gene sequence analysis showed that this strain was affiliated with the genus in the family and presented great similarity with the type strain KMM 6491 (97.9 % sequence similarity). The whole genome of strain HL2-2 comprised 3.6 Mbp with a G+C content of 31.9 mol%. The average nucleotide identity between strain HL2-2 and KMM 6491 was 83.7 %. Growth of the isolated strain was observed from 20-40 °C (optimum, 30 °C), at pH ranged from 5.5 to 8.0 (optimum, pH 6.0) and in the presence of 0-5 % (w/v) NaCl (optimum, 0-2 %). The major fatty acids (>10 % of the total) were C, iso-C and the predominant menaquinone was MK-6. The combined phylogenetic, physiological and chemotaxonomic analysis show that the strain HL2-2 represents a novel species belonging to the genus , for which the name sp. nov. is proposed, and which the type strain is HL2-2 (=CICC 24857=KCTC 72882).
一株革兰氏阴性、需氧、杆状、不具鞭毛但能滑行运动的细菌 HL2-2 从中国的褐藻表面分离得到。16S rRNA 基因序列分析表明，该菌株属于黄杆菌科的 属，与模式菌株 KMM 6491（97.9%序列相似性）具有很高的相似性。菌株 HL2-2 的全基因组由 3.6 Mbp 组成，G+C 含量为 31.9mol%。HL2-2 菌株与 KMM 6491 之间的平均核苷酸同一性为 83.7%。分离株的生长温度为 20-40°C（最佳温度为 30°C），pH 值范围为 5.5-8.0（最佳 pH 值为 6.0），NaCl 浓度为 0-5%（w/v）（最佳浓度为 0-2%）。主要脂肪酸（占总脂肪酸的>10%）为 C、iso-C，主要的menaquinone 为 MK-6。综合系统发育、生理和化学分类学分析表明，该菌株 HL2-2 代表一个属于 属的新物种，提议采用新种名 sp. nov.，其模式菌株为 HL2-2（=CICC 24857=KCTC 72882）。
