do Nascimento José Eranildo Teles, Rodrigues Ana Livya Moreira, de Lisboa Daniele Silva, Liberato Hortência Ribeiro, Falcão Maria José Cajazeiras, da Silva Cecília Rocha, Nobre Júnior Hélio Vitoriano, Braz Filho Raimundo, de Paula Junior Valdir Ferreira, Alves Daniela Ribeiro, de Morais Selene Maia
Programa de Pós-Graduação em Ciências Veterinárias, Núcleo de Pesquisa em Sanidade Animal, Universidade Estadual do Ceará, Fortaleza, CE, Brazil.
Instituto Federal do Ceará, IFCE, Campus Itapipoca, Itapipoca, CE, Brazil.
Evid Based Complement Alternat Med. 2018 Nov 7;2018:1748487. doi: 10.1155/2018/1748487. eCollection 2018.
(Gardner) Engl is popularly used for wound healing. This study describes the main chemical compounds present in extracts of and evaluates their biological potential by investigating antifungal, antioxidant, and anticholinesterase activities. The action mechanism of main antifungal compound was investigated by molecular docking using the enzyme sterol 14- demethylase, CYP51, required for ergosterol biosynthesis. The seeds and leaves were extracted with ethanol in a Soxhlet apparatus and by maceration, respectively. Both extracts were subjected to silica gel column chromatography for isolation of main constituents, followed by purification in sephadex. The structures of compounds were established by H and C-NMR spectroscopy and identified by comparison with literature data as amentoflavone and kaempferol 3--rutinoside, respectively. The antioxidant activities of the extracts were determined by the DPPH and ABTS free radical inhibition methods. In general, the extracts with the highest antioxidant activity corresponded to those with higher content of phenolic compounds and flavonoids. The ethanol extracts and two isolated compounds presented relevant antifungal activity against several strains. The findings revealed that the compound amentoflavone coupled with the CYP450 protein due to the low energy stabilization (-9.39 kcal/mol), indicating a possible mechanism of action by inhibition of the ergosterol biosynthesis of fungi.
（加德纳）英语通常用于伤口愈合。本研究描述了[植物名称]提取物中存在的主要化学成分，并通过研究抗真菌、抗氧化和抗胆碱酯酶活性来评估它们的生物学潜力。使用麦角甾醇生物合成所需的酶甾醇14-脱甲基酶CYP51通过分子对接研究了主要抗真菌化合物的作用机制。种子和叶子分别在索氏提取器中用乙醇提取和通过浸渍法提取。两种提取物都经过硅胶柱色谱法分离主要成分，然后在葡聚糖凝胶中纯化。通过氢核磁共振光谱和碳核磁共振光谱确定化合物的结构，并分别与文献数据比较鉴定为穗花杉双黄酮和山柰酚3-芸香糖苷。提取物的抗氧化活性通过DPPH和ABTS自由基抑制方法测定。一般来说，抗氧化活性最高的提取物对应于酚类化合物和黄酮类化合物含量较高的提取物。乙醇提取物和两种分离的化合物对几种[真菌名称]菌株具有相关的抗真菌活性。研究结果表明，穗花杉双黄酮由于低能量稳定性（-9.39千卡/摩尔）与CYP450蛋白结合，表明可能通过抑制[真菌名称]真菌的麦角甾醇生物合成发挥作用机制。
