DNRF and Villum Fonden Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, IDUN, Department of Micro- and Nanotechnology , Technical University of Denmark , Kgs. Lyngby 2800 , Denmark.
ACS Sens. 2018 Dec 28;3(12):2492-2498. doi: 10.1021/acssensors.8b00887. Epub 2018 Dec 12.
Practical implementation of surfaced enhanced Raman spectroscopy (SERS) sensing is hindered by complexity of real-life samples, which often requires long and costly pretreatment and purification. Here, we present a novel nanopillar-assisted SERS chromatography (NPC-SERS) method for simultaneous quantitation of target molecules and analysis of complex, multicomponent fluids, e.g., human urine spiked with a model drug paracetamol (PAR). Gold-coated silicon nanopillar (AuNP) SERS substrates and a centrifugal microfluidic platform are tactfully combined, which allows (i) a precise and fully automated sample manipulation and (ii) spatial separation of different molecular species on the AuNP substrate. The NPC-SERS technique provides a novel approach for wetting the stationary phase (AuNP) using the "wicking effect", and thus minimizes dilution of analytes. Separation of PAR and the main human urine components (urea, uric acid, and creatinine) has been demonstrated. Quantitative detection of PAR with ultrawide linear dynamic range (0-500 ppm) is achieved by analyzing the spreading profiles of PAR on the AuNP surface. NPC-SERS transforms SERS into a sensing technique with general applicability, facilitating rapid and quantitative detection of analytes in complex biofluids, such as saliva, blood, and urine.
表面增强拉曼光谱（SERS）传感的实际应用受到实际样品复杂性的阻碍，这些样品通常需要耗时且昂贵的预处理和纯化。在这里，我们提出了一种新颖的纳米柱辅助 SERS 色谱（NPC-SERS）方法，用于同时定量目标分子和分析复杂的多组分流体，例如用人尿液中添加的模型药物扑热息痛（PAR）进行掺杂。巧妙地结合了金涂硅纳米柱（AuNP）SERS 基底和离心微流控平台，这使得（i）能够精确且全自动地进行样品操作，（ii）能够在 AuNP 基底上实现不同分子物种的空间分离。NPC-SERS 技术提供了一种使用“芯吸作用”润湿固定相（AuNP）的新方法，从而最大限度地减少了分析物的稀释。已经证明了使用 NPC-SERS 可以分离 PAR 和主要的人尿成分（尿素、尿酸和肌酐）。通过分析 PAR 在 AuNP 表面的扩散分布，可以实现对 PAR 的超宽线性动态范围（0-500ppm）的定量检测。NPC-SERS 将 SERS 转化为一种具有普遍适用性的传感技术，便于快速定量检测唾液、血液和尿液等复杂生物流体中的分析物。
