Emergent Bioengineering Materials Research Team , RIKEN Center for Emergent Matter Science (CEMS) , 2-1 Hirosawa, Wako , Saitama 351-0198 , Japan.
Graduate School of Science and Engineering , Saitama University , 255 Shimo-Okubo, Sakura-ku, Saitama City , Saitama 338-8570 , Japan.
J Am Chem Soc. 2018 Dec 26;140(51):17956-17961. doi: 10.1021/jacs.8b09362. Epub 2018 Dec 10.
Hybrid assemblies composed of phospholipids and amphiphilic polymers have been investigated previously as a biomimetic model of biological cells. However, these studies focused on the functions of polymers in a sea of membrane lipids. Here, we prepared a highly stable peptide-lipid hybrid vesicle from a combination of an amphiphilic polypeptide and the phospholipid, 1,2-dimyristoyl- sn-glycero-3-phosphocholine, with a mixing molar ratio of 1:1. The phase-separated structure of the hybrid vesicle was demonstrated by fluorescence resonance energy transfer analysis. The lipid domain of the hybrid vesicle had a phase-transition temperature of 38 °C and allowed the permeation of a hydrophilic molecule, fluorescein isothiocyanate-labeled polyethylene glycol ( M: 2000), above 38 °C. The designed peptide-lipid hybrid vesicle and a "lipidic gate" are a promising tool for smart drug delivery.
先前已有研究将由磷脂和两亲聚合物组成的混合组装体作为生物细胞的仿生模型进行探讨。然而，这些研究主要关注聚合物在膜脂海洋中的功能。在此，我们通过将两亲性多肽与磷脂 1,2-二肉豆蔻酰基- sn-甘油-3-磷酸胆碱以摩尔比 1:1 混合，制备了一种高度稳定的肽-脂混合囊泡。通过荧光共振能量转移分析证明了混合囊泡的相分离结构。混合囊泡的脂质域具有 38°C 的相转变温度，允许亲水分子，荧光素异硫氰酸酯标记的聚乙二醇（分子量：2000）在 38°C 以上渗透。设计的肽-脂混合囊泡和“脂质门”是智能药物输送的有前途的工具。
