Sun Yu-Tong, Zhou Nan, Wang Bao-Jun, Liu Xiao-Dong, Jiang Cheng-Ying, Ge Xin, Liu Shuang-Jiang
1College of Life Science, Hebei University, Baoding 071002, PR China.
2State Key Laboratory of Microbial Resources, and Environmental Microbiology Research Center (EMRC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2019 Feb;69(2):404-410. doi: 10.1099/ijsem.0.003158. Epub 2018 Dec 12.
A polyphasic study was conducted to characterize an obligately anaerobic bacterial strain, S15, that was isolated from Okinawa Trough sediment. Strain S15 was Gram-stain-negative, non-motile and rod-shaped. Spores were not observed. Strain S15 grew anaerobically at 20-35 °C (optimum at 25-30 °C) and at pH range of 6.0-8.5 (optimum at 7.5). Analysis of 16S rRNA gene sequences showed that strain S15 was phylogenetically related to Vallitalea guaymasensis Ra1766G1 (94.0 %) and Vallitalea pronyensis FatNI3 (93.1 %). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The predominant fatty acids of strain S15 were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The draft genome was 5.86 Mb with a DNA G+C content of 33.9 mol%. A total of 5285 genes were predicted and, of those, 4669 genes were annotated. The genome data supported the result that strain S15 assimilated various carbon sources. On the basis of unique phenotypic, chemotaxonomic and phylogenetic comparisons, strain S15 is proposed to represent a novel species within the genus Vallitalea, and the name Vallitaleaokinawensis sp. nov. is proposed. The type strain is S15=CGMCC 1.5231=KCTC 15675.
开展了一项多阶段研究，以表征从冲绳海槽沉积物中分离出的专性厌氧细菌菌株S15。菌株S15革兰氏染色阴性，无运动性，呈杆状。未观察到孢子。菌株S15在20-35°C（最适温度为25-30°C）和pH值6.0-8.5（最适pH值为7.5）的条件下厌氧生长。16S rRNA基因序列分析表明，菌株S15在系统发育上与瓜伊马斯谷粒菌Ra1766G1（94.0%）和普氏谷粒菌FatNI3（93.1%）相关。主要极性脂为双磷脂酰甘油、磷脂酰甘油和糖脂。菌株S15的主要脂肪酸为异C15:0、anteiso-C15:0、异C16:0和C16:0。基因组草图为5.86 Mb，DNA G+C含量为33.9 mol%。共预测到5285个基因，其中4669个基因得到注释。基因组数据支持菌株S15能同化多种碳源这一结果。基于独特的表型、化学分类和系统发育比较，菌株S15被提议代表谷粒菌属内的一个新物种，并提议命名为冲绳谷粒菌（Vallitaleaokinawensis sp. nov.）。模式菌株为S15=CGMCC 1.5231=KCTC 15675。
