Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.
Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288, Marseille, cedex 09, France.
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1160-1165. doi: 10.1099/ijs.0.055756-0. Epub 2014 Jan 9.
A novel thermotolerant, anaerobic, Gram-stain-positive, spore-forming bacterium was isolated from a hydrothermal chimney in Prony Bay, New Caledonia. This strain, designated FatNI3(T), grew at 15-55 °C (optimum 30 °C) and at pH 5.8-8.9 (optimum 7.7). It was slightly halophilic, requiring at least 0.5 % NaCl for growth (optimum 2.5-3.0 %), and was able to grow at up to 6 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Growth of strain FatNI3(T) was inhibited in the presence of sulfite (2 mM) or nitrite (2 mM). Strain FatNI3(T) fermented cellobiose, glucose, mannose, maltose, sucrose, galactose, lactose, ribose, fructose, rhamnose, raffinose, xylose, yeast extract, peptone and biotrypticase. The main fermentation products from glucose metabolism were acetate, ethanol, H2 and CO2. The predominant cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, and unknown glycolipids and phospholipids. The G+C content of the genomic DNA was 36.6 mol%. On the basis of phylogenetic and physiological properties, strain FatNI3(T) ( = DSM 25904 = JCM 18391) belonging to the phylum Firmicutes, class Clostridia, order Clostridiales, is proposed as the type strain of a novel species of the genus Vallitalea, for which the name Vallitalea pronyensis sp. nov. is proposed.
一种新型耐热、厌氧、革兰氏阳性、芽孢形成细菌，从新喀里多尼亚 Prony 湾的热液烟囱中分离出来。该菌株被命名为 FatNI3(T)，可在 15-55°C（最佳 30°C）和 pH5.8-8.9（最佳 7.7）的条件下生长。它略带嗜盐性，生长至少需要 0.5%的 NaCl（最佳 2.5-3.0%），并且能够在高达 6%的 NaCl 中生长。硫酸盐、硫代硫酸盐、元素硫、亚硫酸盐、硝酸盐和亚硝酸盐不能作为末端电子受体。菌株 FatNI3(T)在亚硫酸盐（2 mM）或亚硝酸盐（2 mM）存在下的生长受到抑制。菌株 FatNI3(T)发酵纤维二糖、葡萄糖、甘露糖、麦芽糖、蔗糖、半乳糖、乳糖、核糖、果糖、鼠李糖、棉子糖、木糖、酵母提取物、蛋白胨和胰蛋白酶。葡萄糖代谢的主要发酵产物为乙酸、乙醇、H2 和 CO2。主要的细胞脂肪酸为 iso-C15：0 和 anteiso-C15：0。主要的极性脂类由双磷脂酰甘油、磷脂酰甘油和未知的糖脂和磷脂组成。基因组 DNA 的 G+C 含量为 36.6 mol%。基于系统发育和生理特性，菌株 FatNI3(T)（ = DSM 25904 = JCM 18391）属于厚壁菌门、梭菌纲、梭菌目，建议作为 Vallitalea 属的一个新型种的模式株，该种命名为 Prony 湾瓦尔蒂拉氏菌（Vallitalea pronyensis）。
