Cao Peng, Wang Ying, Sun Peng, Li Chenxu, Zhao Junwei, Jiang Shanwen, Zhang Yan, Wang Xiangjing, Xiang Wensheng
1Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Xiangfang, Harbin 150030, PR China.
2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2019 Feb;69(2):316-321. doi: 10.1099/ijsem.0.003113. Epub 2018 Dec 12.
A novel actinobacterium, designated strain NEAU-YG30, was isolated from the rhizosphere soil of lettuce collected in Heilongjiang province, north-east China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-YG30 belongs to the genus Nonomuraea, and shared highest sequence similarity to Nonomuraea muscovyensis KCTC 29233 (97.9 %) and Nonomuraea indica CCTCC AA 209050 (97.6 %). The major menaquinone was identified as MK-9(H4). The major fatty acids were 10-methyl C17 : 0, C17 : 0, iso-C16 : 0 2-OH, iso-C16 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids. The genomic DNA G+C content of strain NEAU-YG30 was 70.5 mol%. DNA-DNA hybridization values between them were less than 70 %. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-YG30 can be characterized to represent a novel species of the genus Nonomuraea, for which the name Nonomuraealactucae sp. nov. is proposed. The type strain is NEAU-YG30 (=JCM 32549=CGMCC 4.7506).
从中国东北黑龙江省采集的生菜根际土壤中分离出一株新型放线菌，命名为NEAU-YG30菌株。采用多相分类法对该菌株的分类地位进行了研究。基于16S rRNA基因序列分析，NEAU-YG30菌株属于野野村氏菌属，与番鸭野野村氏菌KCTC 29233（97.9%）和印度野野村氏菌CCTCC AA 209050（97.6%）的序列相似性最高。主要甲基萘醌被鉴定为MK-9（H4）。主要脂肪酸为10-甲基C17 : 0、C17 : 0、异-C16 : 0 2-OH、异-C16 : 0和C16 : 0。极性脂类为双磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰肌醇、一种未鉴定的磷脂和三种未鉴定的脂类。NEAU-YG30菌株的基因组DNA G+C含量为70.5 mol%。它们之间的DNA-DNA杂交值小于70%。基于表型、基因型和系统发育数据，NEAU-YG30菌株可被鉴定为野野村氏菌属的一个新物种，为此提出新名称野野村氏生菜菌（Nonomuraealactucae sp. nov.）。模式菌株为NEAU-YG30（=JCM 32549=CGMCC 4.7506）。
