Department of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, Osijek 31000, Croatia.
Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr. 20, Tulln 3430, Austria.
Toxins (Basel). 2018 Dec 10;10(12):528. doi: 10.3390/toxins10120528.
is the most important mycotoxin-producing fungus involved in the global episodes of aflatoxin B₁ contamination of crops at both the pre-harvest and post-harvest stages. However, in order to effectively control aflatoxin contamination in crops using antiaflatoxigenic and/or antifungal compounds, some of which are photosensitive, a proper understanding of the photo-sensitive physiology of potential experimental strains need to be documented. The purpose of the study is therefore to evaluate the effect of visible (VIS) light illumination on growth and conidiation, aflatoxin production ability and modulation of oxidative status during in vitro experiment. Aflatoxigenic strain was inoculated in aflatoxin-inducing YES media and incubated under three different VIS illumination regimes during a 168 h growth period at 29 °C. VIS illumination reduced mycelia biomass yield, both during growth on plates and in liquid media, promoted conidiation and increased the aflatoxin production. Furthermore, aflatoxin production increased with increased reactive oxidative species (ROS) levels at 96 h of growth, confirming illumination-driven oxidative stress modulation activity on cells.
是最主要的产毒真菌，涉及到在收获前和收获后阶段全球范围内的作物黄曲霉毒素 B₁污染事件。然而，为了使用抗黄曲霉和/或抗真菌化合物有效控制作物中的黄曲霉污染，其中一些是光敏感的，需要记录对潜在实验菌株的光敏感生理学的适当理解。因此，本研究的目的是评估可见光（VIS）光照对生长和产孢、产黄曲霉毒素能力以及体外实验期间氧化状态调节的影响。将产黄曲霉毒素的菌株接种在黄曲霉诱导的 YES 培养基中，并在 29°C 下的 168 小时生长期间在三种不同的 VIS 光照条件下进行培养。VIS 光照减少了平板和液体培养基上生长期间的菌丝体生物量产量，促进了产孢并增加了黄曲霉毒素的产生。此外，产黄曲霉毒素的产生随着生长 96 小时时活性氧（ROS）水平的增加而增加，证实了光照驱动的对细胞的氧化应激调节活性。
