Signer E N, Anzenberger G, Jeffreys A J
Department of Genetics, University of Leicester, LE1 7RH, Leicester, England.
Anthropologisches Institut, Universität Zürich-Irchel, 8057, Zürich, Switzerland.
Primates. 2000 Jan;41(1):49-61. doi: 10.1007/BF02557461.
Marmosets normally produce dizygotic twins sharing placental blood vessels and exchanging bone marrow cells. Each individual is therefore likely to be a blood chimaera. To date, marmosets had only been DNA fingerprinted using blood samples and probes 33.6 and 33.15, resulting in highly similar fingerprints among litter mates and little variation between other individuals, thereby limiting this method's use for individual identification and parentage testing. In this study, novel probes were applied to detect greater polymorphism and to produce individual-specific DNA fingerprints. As expected, blood DNA profiles of twins and triplets were virtually identical, confirming chimaerism in this tissue and identifying litter mates. Furthermore, these profiles were sufficiently variable to distinguish between sibs from different litters and between all other individuals. To produce individual-specific DNA fingerprints, the use of DNA extracted from tissues poor in leukocytes was essential. The findings demonstrate that, despite extensive blood chimaerism, marmoset colonies can be effectively DNA fingerprinted for indicidual identification, zygosity testing, and relationship studies.
狨猴通常会产下异卵双胞胎，它们共享胎盘血管并交换骨髓细胞。因此，每只个体都可能是血液嵌合体。到目前为止，狨猴仅使用血液样本以及探针33.6和33.15进行过DNA指纹识别，结果同窝幼仔之间的指纹高度相似，其他个体之间的差异很小，从而限制了这种方法在个体识别和亲子鉴定中的应用。在本研究中，应用了新型探针来检测更高的多态性并产生个体特异性的DNA指纹。正如预期的那样，双胞胎和三胞胎的血液DNA图谱几乎相同，证实了该组织中的嵌合现象并识别出同窝幼仔。此外，这些图谱的变异性足以区分来自不同窝的同胞以及所有其他个体。为了产生个体特异性的DNA指纹，使用从白细胞含量低的组织中提取的DNA至关重要。研究结果表明，尽管存在广泛的血液嵌合现象，但狨猴群体仍可有效地进行DNA指纹识别，用于个体识别、合子性检测和亲缘关系研究。
