Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax, 3038, Tunisia.
Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax, 3038, Tunisia.
Carbohydr Polym. 2019 Feb 15;206:380-388. doi: 10.1016/j.carbpol.2018.11.020. Epub 2018 Nov 13.
A novel water-soluble polysaccharide named PLP1 was successfully isolated from root barks of Periploca laevigata by hot water extraction and further purified by DEAE-sepharose chromatography. PLP1 has a relative molar mass of 5.57 10 g/mol determined by Ultra-high performance liquid chromatography (UHPLC). The PLP1 structure was investigated by chemical and instrumental analysis including gas chromatography mass spectrometry (GC-MS), methylation analysis, Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic spectroscopy (H NMR and C NMR). Results showed that PLP1 was mainly composed of glucose, galactose, mannose and glucuronic acid in a molar percent of 62.9, 19.4, 11.8 and 5.9, respectively. The backbone of PLP1 was composed of 1,3-β-d-Glcp, 1,3,4-α-d-Glcp, 1,4-α-d-Manp, 1,6-α-d-Manp, 1,3-α-d-Galp and 1,6-β-d-Galp. The thermogravimetry analysis (TGA) and the differential scanning calorimetry (DSC) were used and showed that PLP1 has good thermal stability under 229 °C. Moreover, the purified polysaccharide demonstrated an appreciable in vitro antioxidant potential and high antibacterial activity against several Gram (+) and Gram (-) strains. These finding suggested that PLP1 might be suitable for use as functional foods and as potential therapeutic agents.
从杠柳的根皮中成功提取到一种新型水溶性多糖 PLP1，该多糖经热水抽提和 DEAE-琼脂糖柱层析进一步纯化。通过超高效液相色谱（UHPLC）确定 PLP1 的相对摩尔质量为 5.57×10^4g/mol。通过包括气相色谱-质谱联用（GC-MS）、甲基化分析、傅里叶变换红外（FT-IR）光谱和核磁共振光谱（H NMR 和 C NMR）在内的化学和仪器分析，对 PLP1 的结构进行了研究。结果表明，PLP1 主要由葡萄糖、半乳糖、甘露糖和葡萄糖醛酸组成，摩尔百分比分别为 62.9%、19.4%、11.8%和 5.9%。PLP1 的骨架由 1,3-β-d-Glcp、1,3,4-α-d-Glcp、1,4-α-d-Manp、1,6-α-d-Manp、1,3-α-d-Galp 和 1,6-β-d-Galp 组成。热重分析（TGA）和差示扫描量热法（DSC）表明，PLP1 在 229°C 以下具有良好的热稳定性。此外，纯化后的多糖具有显著的体外抗氧化潜力和对几种革兰氏阳性和革兰氏阴性菌株的高抗菌活性。这些发现表明，PLP1 可能适合用作功能性食品和潜在的治疗剂。
