College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, China.
Int J Biol Macromol. 2019 Sep 1;136:607-615. doi: 10.1016/j.ijbiomac.2019.06.117. Epub 2019 Jun 17.
An acidic polysaccharide (SAZMP3) was isolated from jujube fruit residues by alkaline extraction and purified with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography. The structural characterizations of SAZMP3 were determined by high-performance gelpermeation chromatography, gas chromatography, Fourier transform infrared spectroscopy, methylation analysis, NMR spectroscopy, scanning electron microscopy, and atomic force microscopy. The results determined SAZMP3 was an acidic polysaccharide mainly contained rhamnose, galactose, and galacturonic acid with a molecular weight of 9.37 kDa and its backbone might mainly be composed of 1,4-α-D-GalAp with side chains of 1,3-β-D-Galp, 1,3,5-linked Araf, 1,2,4-α-L-Rhap and terminals of 1-linked Araf, 1-linked Rhap, 1-linked Galp by methylation and NMR analysis. Besides, SAZMP3 has shown potential antioxidant activity in scavenging free radicals suggesting that it is an antioxidant agent, which might contribute to the functional food qualities of jujube fruit.
从红枣残渣中经碱性提取、DEAE-葡聚糖快速流动层析柱和 Sephacryl S-300 层析柱纯化，得到一种酸性多糖（SAZMP3）。采用高效凝胶渗透色谱、气相色谱、傅里叶变换红外光谱、甲基化分析、NMR 光谱、扫描电子显微镜和原子力显微镜等方法对 SAZMP3 的结构特征进行了测定。结果表明，SAZMP3 是一种酸性多糖，主要由鼠李糖、半乳糖和半乳糖醛酸组成，分子量为 9.37 kDa，其骨架可能主要由 1,4-α-D-GalAp 组成，侧链由 1,3-β-D-Galp、1,3,5-连接的 Araf、1,2,4-α-L-Rhap 和末端的 1-连接的 Araf、1-连接的 Rhap、1-连接的 Galp 组成，通过甲基化和 NMR 分析。此外，SAZMP3 在清除自由基方面表现出潜在的抗氧化活性，表明其是一种抗氧化剂，这可能有助于红枣的功能性食品品质。
Int J Biol Macromol. 2019-11-16
Int J Biol Macromol. 2012-12-19
Int J Biol Macromol. 2017-2
Zotero 插件