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载银创伤敷料的多层系统包被体外药物释放和抗菌活性评价

In vitro evaluation of drug release and antibacterial activity of a silver-loaded wound dressing coated with a multilayer system.

机构信息

Univ. Lille, INSERM, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France.

Univ. Lille, INSERM, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France; Univ. Lille, INSERM, CHU Lille, U995- LIRIC - Lille Inflammation Research International Center, F-59000 Lille, France.

出版信息

Int J Pharm. 2019 Feb 10;556:301-310. doi: 10.1016/j.ijpharm.2018.12.018. Epub 2018 Dec 13.

Abstract

The goal of the study was to elaborate an antibacterial silver wound dressing covered by a protective coating that would prevent silver diffusion toward skin without losing its biocide properties. Therefore, non woven polyethyleneterephtalate (PET) textiles were pre-treated by two types of polysaccharides - chitosan and cyclodextrin - both crosslinked with citric acid by a pad/dry/cure process. Both types of resulting thermofixed textiles carrying the citrate crosslinks were then impregnated in silver solution followed by a thermal treatment and were finally coated by Layer-by-Layer (L-b-L) deposition of a polyelectrolyte multilayer (PEM) film consisting of anionic water-soluble poly-cyclodextrin and cationic chitosan. The influence of the process parameters was investigated in terms of silver adsorption capacity, PEM system build-up, silver kinetics of release and antibacterial activity. We demonstrate i) the utility of the intermediate thermal treatment step in the reduction of silver leakage in the polyelectrolyte solutions used in the L-b-L process, ii) that silver adsorption on the preliminary thermofixed layers did not affect the PEM system build-up, iii) the slowing down of silver release kinetic thanks to the PEM coating, iv) the preservation of the antibacterial activity despite the PEM coating.

摘要

本研究的目的是开发一种具有抗菌性能的银创伤敷料,其表面覆盖有保护层,以防止银向皮肤扩散,同时保持其杀菌性能。为此,我们采用两种多糖(壳聚糖和环糊精)对非织造聚对苯二甲酸乙二醇酯(PET)纺织品进行预处理,这两种多糖均通过柠檬酸的交联作用形成交联网络。通过轧-烘-焙工艺对预处理后的热固定纺织品进行交联,然后将其浸渍在银溶液中,再进行热处理,最后通过层层(L-b-L)自组装技术在其表面涂覆一层由阴离子型水溶性环糊精和阳离子型壳聚糖组成的聚电解质多层(PEM)膜。研究了工艺参数对银吸附容量、PEM 体系的构建、银释放动力学和抗菌活性的影响。结果表明:i)中间热处理步骤可减少 L-b-L 过程中聚电解质溶液中的银泄漏;ii)初步热固定层上的银吸附不影响 PEM 体系的构建;iii)PEM 涂层可减缓银的释放动力学;iv)尽管经过 PEM 涂层处理,仍能保持抗菌活性。

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