Craig P S, Macpherson C N, Watson-Jones D L, Nelson G S
Department of Parasitology, Liverpool School of Tropical Medicine, UK.
Trans R Soc Trop Med Hyg. 1988;82(2):268-74. doi: 10.1016/0035-9203(88)90445-2.
A species-specific indirect immunofluorescence test using an anti-Echinococcus oncosphere monoclonal antibody (EgOH6-4E5) was applied to identify Echinococcus oncospheres released from taeniid eggs collected in environmental soil and water samples, and from perianal or faecal samples of naturally infected dogs, in northern Turkana, Kenya. The specificity of immunodetection of Echinococcus eggs by specific fluorescence of Echinococcus oncospheres from naturally infected dogs was 100% when compared to Taenia hydatigena infections, and a sensitivity of 73% was obtained in the detection of dogs infected with Echinococcus using perianal Scotch tape swabs. Taeniid eggs were recovered from various soil samples inside Turkana manyattas (settlements) and from waterhole samples. Some oncospheres obtained from taeniid eggs recovered from all sampled areas, but particularly from inside Turkana huts (akai) and from water samples from open waterholes used by the people and their livestock, reacted positively with the Echinococcus 4E5 monoclonal antibody. The potential importance of contamination of such sites with Echinococcus eggs is discussed in relation to the transmission of echinococcosis in this hyperendemic region of northern Kenya.
在肯尼亚图尔卡纳北部，应用一种使用抗细粒棘球绦虫六钩蚴单克隆抗体（EgOH6 - 4E5）的种特异性间接免疫荧光试验，来鉴定从环境土壤和水样以及自然感染犬的肛周或粪便样本中收集的带绦虫卵释放出的细粒棘球绦虫六钩蚴。与泡状带绦虫感染相比，通过自然感染犬的细粒棘球绦虫六钩蚴的特异性荧光对细粒棘球绦虫虫卵进行免疫检测的特异性为100%，并且使用肛周透明胶带拭子检测感染细粒棘球绦虫的犬时，灵敏度为73%。在图尔卡纳的许多atta（定居点）内的各种土壤样本以及水坑样本中都发现了带绦虫卵。从所有采样区域收集的带绦虫卵中获得的一些六钩蚴，特别是从图尔卡纳房屋（akai）内以及人和他们的牲畜使用的露天水坑的水样中获得的六钩蚴，与细粒棘球绦虫4E5单克隆抗体发生了阳性反应。结合肯尼亚北部这个高度流行地区棘球蚴病的传播，讨论了这些场所被细粒棘球绦虫虫卵污染的潜在重要性。
