Craig P S, Macpherson C N, Nelson G S
Am J Trop Med Hyg. 1986 Jan;35(1):152-8. doi: 10.4269/ajtmh.1986.35.152.
A relatively simple and specific test has been developed to distinguish eggs of Echinococcus from those of other morphologically identical taeniid species. A specific anti-Echinococcus oncosphere monoclonal antibody was produced which binds in an indirect immunofluorescence test to egg-derived oncospheres of E. granulosus but not to those of other taeniid species, such as Taenia hydatigena, T. saginata, T. pisiformis, T. ovis, T. multiceps, or T. taeniaeformis. Specific fluorescence was obtained with oncospheres of E. granulosus derived from either hatch/activated viable eggs using artificial intestinal fluid or from hypochlorite/detergent treated eggs. The potential use of this test in the study of the transmission of Echinococcus in Turkana, Kenya, is discussed.
已经开发出一种相对简单且特异的检测方法，用于区分棘球绦虫的虫卵与其他形态相同的带绦虫物种的虫卵。制备了一种特异性抗棘球绦虫六钩蚴单克隆抗体，该抗体在间接免疫荧光试验中能与细粒棘球绦虫虫卵衍生的六钩蚴结合，但不与其他带绦虫物种（如泡状带绦虫、牛带绦虫、豆状带绦虫、羊带绦虫、多头带绦虫或带状带绦虫）的六钩蚴结合。使用人工肠液从孵化/激活的活虫卵中获得的细粒棘球绦虫六钩蚴，或从经次氯酸盐/洗涤剂处理的虫卵中获得的细粒棘球绦虫六钩蚴，均可产生特异性荧光。本文讨论了该检测方法在肯尼亚图尔卡纳地区棘球绦虫传播研究中的潜在应用。
